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Series GSE84519 Query DataSets for GSE84519
Status Public on Mar 02, 2017
Title Opposing macrophage-polarization programs show extensive epigenomic and transcriptional cross-talk [ChIP_broadly]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The M1 and the M2 macrophage polarization programs (activated by IFNγ and IL-4, respectively) lie at the opposite edges of a continuum of activation states but are frequently co-activated during co-infections and in cancer despite controlling divergent functional responses. Whether these two programs are mutually exclusive, how they influence each other, and whether one represents the prevailing response, are all open questions. Co-administration of IFNγ and IL-4 exerted complex inhibitory effects over the M1 and M2 programs at the level of both epigenomic and transcriptional changes. Computational data mining and validation analyses revealed the molecular basis of the differential sensitivity of genes and cis-regulatory elements to the antagonistic effects of the opposite stimulus. For instance, while STAT1 and IRF motifs were associated with robust and IL-4-resistant responses to IFNγ, their coexistence with binding sites for some auxiliary transcription factors such as AP-1, generated vulnerability to IL-4-mediated inhibition. These data provide a core mechanistic framework for the integration of signals that control macrophage activation and the starting point for understanding macrophage responses in complex environmental conditions
 
Overall design Analysis of transcriptional and epigenomic changes in mouse macrophages stimulated with different cytokines or their combinations
 
Contributor(s) Piccolo V, Curina A, Genua M, Ghisletti S, Simonatto M, Sabò A, Amati B, Ostuni R, Natoli G
Citation(s) 28288101
Submission date Jul 18, 2016
Last update date May 15, 2019
Contact name Viviana Piccolo
E-mail(s) viviana.piccolo@ieo.it
Organization name European Institute of Oncology (IEO)
Department Department of Experimental Oncology
Lab Gioacchino Natoli Lab
Street address Via Adamello 16
City Milano
State/province Milano
ZIP/Postal code 20139
Country Italy
 
Platforms (1)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
Samples (13)
GSM2241203 ChIP-Seq H3K27ac_UT
GSM2241204 ChIP-Seq H3K27ac_IL4_2h
GSM2241205 ChIP-Seq H3K27ac_IFNg_2h
This SubSeries is part of SuperSeries:
GSE84520 Opposing macrophage-polarization programs show extensive epigenomic and transcriptional cross-talk
Relations
BioProject PRJNA329535
SRA SRP078842

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Supplementary file Size Download File type/resource
GSE84519_RAW.tar 12.5 Mb (http)(custom) TAR (of BED, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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