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Series GSE84396 Query DataSets for GSE84396
Status Public on Jan 01, 2017
Title Analysis of gene expression in TET1 depleted colorectal cancer Colo320DM cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We aimed to analyze the relationship between TET1 and aberrant CpG methylation in colorectal cancer (CRC). We established three stable TET1 knockdown clones and negative control clones of Colo320DM cells, and carried out gene expression analysis with Agilent Human Gene Expression microarray kit.
 
Overall design RNAi-induced TET1 knockdown was accomplished using a BLOCK-iT Pol II miR RNAi Expression Vector kit (Thermo Fisher Scientific). Two sets of oligonucleotides targeting TET1 were purchased from Invitrogen and ligated into a pcDNA6.2-GW/EmGFP-miR vector (Thermo Fisher Scientific). Cells were transfected with each TET1 knockdown vector or a pcDNA6.2-GW/EmGFP-miR-neg control plasmid (Thermo Fisher Scientific), after which they were selected with 1.0 mg/ml G418. GFP-positive colonies were isolated, and knockdown efficiencies were analyzed using RT-qPCR.
 
Contributor(s) Suzuki H
Citation(s) 27977763
Submission date Jul 14, 2016
Last update date Apr 23, 2018
Contact name Hiromu Suzuki
E-mail(s) hsuzuki@sapmed.ac.jp
Phone +81-11-611-2111
Organization name Sapporo Medical University
Department Department of Molecular Biology
Street address S1, W17, Chuo-ku
City Sapporo
State/province Hokkaido
ZIP/Postal code 060-8556
Country Japan
 
Platforms (1)
GPL16699 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Feature Number version)
Samples (12)
GSM2233020 Colo320DM control #1
GSM2233021 Colo320DM control #2
GSM2233022 Colo320DM control #3
This SubSeries is part of SuperSeries:
GSE84400 Analysis of DNA methylation and gene expression in TET1 depleted colorectal cancer cells
Relations
BioProject PRJNA329081

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE84396_RAW.tar 148.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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