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Series GSE8437 Query DataSets for GSE8437
Status Public on Jan 18, 2008
Title IP Staufen2
Organism Homo sapiens
Experiment type Other
Summary In human cells, Staufen2 is a double-stranded RNA-binding protein involved in several cellular functions. Although 51% identical to Staufen1, these proteins are nevertheless found in different RNA particles. In addition, differential splicing events generate Staufen2 isoforms that only differ at their N-terminal extremities. We used a genome wide approach to identify and compare the mRNA targets of mammalian Staufen2 isoforms. The mRNA content of Staufen mRNPs was identified by probing DNA microarrays with probes derived from mRNAs isolated from immunopurified Staufen2-containing complexes following transfection of HEK293T cells with Stau2-HA (59kDa) or Stau2-HA (62kDa) expressors. Our results indicate that 11% of the cellular RNAs expressed in HEK293T cells are found in Stau2-containing mRNPs. There is a predominance of mRNAs involved in cell metabolism, transport, transcription, regulation of cell processes and catalytic activity.
Keywords: Immunoprecipitation, Staufen, microarray
 
Overall design HEK293 cells were transiantly transfected with plasmids coding for Staufen2-HA isoforms. Cell extracts were immunoprecipitated using anti-HA antibodies and co-immunoprecipitated mRNAs were purified and used to hybridize microarrays. As control, cell extract from mock transfected cells were used.
 
Contributor(s) Furic L, Maher-Laporte M, DesGroseillers L
Citation(s) 18094122
Submission date Jul 11, 2007
Last update date Mar 25, 2019
Contact name Luc DesGroseillers
E-mail(s) luc.desgroseillers@umontreal.ca
Organization name Université de Montréal
Department Biochimie
Street address 2900 Edouard Montpetit
City Montréal
State/province Québec
ZIP/Postal code H3T1J4
Country Canada
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (6)
GSM209689 IP Staufen2 59kDa duplicate
GSM209694 IP Staufen2 62kDa duplicate
GSM209697 IP control(2) duplicate
Relations
BioProject PRJNA101503

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8437_RAW.tar 122.5 Mb (http)(custom) TAR (of CEL, CHP, EXP)
Processed data included within Sample table
Processed data provided as supplementary file

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