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| Status |
Public on Feb 09, 2017 |
| Title |
Large-scale microRNA expression profiling identifies retinal miRNA-mRNA signaling pathways underlying form-deprivation myopia in mice |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Development of myopia is associated with large-scale changes in ocular tissue gene expression. Although differential expression of coding genes underlying development of myopia has been a subject of intense investigation, the role of non-coding genes such as microRNAs in the development of myopia is largely unknown. In this study, we explored myopia-associated miRNA expression profiles in the retina and sclera of C57Bl/6J mice with experimentally induced myopia using microarray technology. We found a total of 53 differentially expressed miRNAs in the retina and no differences in miRNA expression in the sclera of C57BL/6J mice after 10 days of visual form deprivation, which induced -6.93 ± 2.44 D (p < 0.000001, n = 12) of myopia. We also identified their putative mRNA targets among mRNAs found to be differentially expressed in myopic retina and potential signaling pathways involved in the development of form-deprivation myopia using miRNA-mRNA interaction network analysis. Analysis of myopia-associated signaling pathways revealed that myopic response to visual form deprivation in the retina is regulated by a small number of highly integrated signaling pathways. Our findings highlight substantial involvement of miRNAs in the regulation of refractive eye development, and in the development of myopia through the retinal gene regulation.
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| Overall design |
Differential expression of microRNAs was analyzed in the retina and sclera of C57BL/6J mice after 10 days of visual form deprivation. Visual form deprivation was carried out from P24 through P34. Three replicates were used per condition, i.e., myopia versus control.
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| Contributor(s) |
Tkatchenko AV, Luo X, Tkatchenko TV, Vaz C, Tanavde VM, Maurer-Stroh S, Zauscher S, Gonzalez P, Young TL |
| Citation(s) |
27622715 |
| |
| Submission date |
Jul 10, 2016 |
| Last update date |
Feb 09, 2017 |
| Contact name |
Andrei V Tkatchenko |
| E-mail(s) |
avt2130@cumc.columbia.edu
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| Phone |
212-342-5135
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| Organization name |
Columbia University
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| Department |
Ophthalmology
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| Street address |
160 Ft Washington Ave
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
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| Platforms (1) |
| GPL22134 |
Agilent-029152 Unrestricted Mouse miRNA Microarray 028772 (miRBase release 18 miRNA ID version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA328402 |
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