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Series GSE84019 Query DataSets for GSE84019
Status Public on Feb 15, 2017
Title Gene expression of liver sinusoidal endothelial cells from an 8-week-old WT and ob/ob mouse
Organism Mus musculus
Experiment type Expression profiling by array
Summary Obesity may promote myeloid cell accumulation in liver. Intravital liver tissue imaging revealed that the number of myeloid cells attached to sinusoidal wall significantly increased in ob/ob mice. To assess the pathophysiological roll of liver sinusoidal endothelial cells (LSEC) in obese livers, we performed microarray analysis in LSEC isolated from an 8-week-old wild-type and ob/ob mouse. Pathway analysis in microarray data shows genes related to cell adhesion, chemokine signaling and leukocyte transendothelial migration are upregulated in LSEC from ob/ob.
Overall design Liver sinusoidal endothelial cells were isolated by collagenage digestion of livers from an 8-week-old male wild-type and ob/ob mouse. Cells were cultured overnight in DMEM containing 10% FBS.
Contributor(s) Miyachi Y, Yamaguchi S, Tsuchiya K, Ogawa Y
Citation(s) 28297678
Submission date Jul 05, 2016
Last update date Jan 19, 2018
Contact name Yasutaka Miyachi
Organization name Tokyo Medical and Dental University
Department Molecular Endocrinology and Metabolism
Street address 1-5-45 Yushima
City Bunko-ku
State/province Tokyo
ZIP/Postal code 1138510
Country Japan
Platforms (1)
GPL10333 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Feature Number version)
Samples (2)
GSM2225724 WT LSEC
GSM2225726 ob/ob LSEC
BioProject PRJNA327826

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE84019_RAW.tar 24.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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