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Series GSE84013 Query DataSets for GSE84013
Status Public on Dec 11, 2017
Title Reprogrammed neurons from adult dermal fibroblasts of patients with Huntington's disease
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Reprogrammed neurons from adult dermal fibroblasts of patients with Huntington's disease
Overall design De-identified human adult dermal fibroblasts from patients with Huntington's disease or age- and gender-matched healthy controls were obtained from the Coriell Institute for Medical Research Biorepository. These fibroblast samples were transduced with lentivirus to express the brain enriched microRNAs, miR-9/9* and miR-124 and striatal-enriched transcription factors BCL11B (Also known as CTIP2), DLX1, DLX2, and MYT1L to directly convert fibroblasts into striatal neurons as previously established (Victor et al., Neuron 2014; Richner et al., Nat. Prot. 2015). Total mRNA from reprogrammed neurons and non-converted fibroblasts was collected and prepared for RNA-seq analysis 32 days after viral transduction.
Contributor(s) Victor M, Yoo A
Citation(s) 29403030
Submission date Jul 05, 2016
Last update date Jul 08, 2019
Contact name Bo Zhang
Phone 1-314-362-4757
Organization name Washington University School of Medicine
Department Developmental Biology
Street address 660 S. Euclid Avenue
City Saint Louis
State/province MO
ZIP/Postal code 63110
Country USA
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (32)
GSM2225648 HD1-Fib-A
GSM2225649 HD1-Fib-B
GSM2225650 HD1-MSN-A
BioProject PRJNA327817
SRA SRP077915

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE84013_RAW.tar 5.1 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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