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| Status |
Public on Dec 11, 2017 |
| Title |
Reprogrammed neurons from adult dermal fibroblasts of patients with Huntington's disease |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Reprogrammed neurons from adult dermal fibroblasts of patients with Huntington's disease
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| Overall design |
De-identified human adult dermal fibroblasts from patients with Huntington's disease or age- and gender-matched healthy controls were obtained from the Coriell Institute for Medical Research Biorepository. These fibroblast samples were transduced with lentivirus to express the brain enriched microRNAs, miR-9/9* and miR-124 and striatal-enriched transcription factors BCL11B (Also known as CTIP2), DLX1, DLX2, and MYT1L to directly convert fibroblasts into striatal neurons as previously established (Victor et al., Neuron 2014; Richner et al., Nat. Prot. 2015). Total mRNA from reprogrammed neurons and non-converted fibroblasts was collected and prepared for RNA-seq analysis 32 days after viral transduction.
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| Contributor(s) |
Victor M, Yoo A |
| Citation(s) |
29403030 |
| Submission date |
Jul 05, 2016 |
| Last update date |
Jul 08, 2019 |
| Contact name |
Bo Zhang |
| E-mail(s) |
zhangbo@wusm.wustl.edu
|
| Phone |
1-314-362-4757
|
| Organization name |
Washington University School of Medicine
|
| Department |
Developmental Biology
|
| Street address |
660 S. Euclid Avenue
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (32)
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| Relations |
| BioProject |
PRJNA327817 |
| SRA |
SRP077915 |
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