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Series GSE83960 Query DataSets for GSE83960
Status Public on Feb 24, 2017
Title ASXL1 Interacts with the Cohesin Complex to Maintain Chromatid Separation and Gene Expression for Normal Hematopoiesis [ChIP-Seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary ASXL1 is frequently mutated in a spectrum of myeloid malignancies with poor prognosis. Loss of Asxl1 leads to myelodysplastic syndrome-like disease in mice, however, the underlying molecular mechanisms remain unclear. Here, we report that ASXL1 interacts with the cohesin complex, which has been shown to guide sister chromotid segregation and to regulate gene expression. Loss of Asxl1 impairs the cohesin function as reflected by an impaired telophase chromatid disjunction in hematopoietic cells. ChIP-seq data revealed that ASXL1, RAD21 and SMC1A share 93% of genomic binding sites at promoter regions in lineage-cKit+ (LK) cells. We have showed that loss of Asxl1 reduced the genome binding of RAD21 and SMC1A, and altered the expression of ASXL1/cohesin target genes in LK cells. Our study underscores the ASXL1-cohesin interaction as a novel means to maintain normal sister chromatid separation and to regulate gene expression in hematopoietic cells.
Overall design The DEG genes' relation with the changes of ASXL1 peaks and Cohesin peaks changes
Contributor(s) Li Z, Zhang P, Yang F
Citation(s) 28116354
Submission date Jul 01, 2016
Last update date May 15, 2019
Contact name Peng Zhang
Organization name University of Miami Miller School of Medicine
Lab Feng-Chun Yang
Street address 1011 NW 15th Street
City Miami
State/province FL
ZIP/Postal code 33136
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (6)
GSM2224545 ASXL1_WT_ChIPseq
GSM2224546 SMC1A_WT_ChIPseq
GSM2224547 SMC1A_KO_ChIPseq
This SubSeries is part of SuperSeries:
GSE83962 ASXL1 Interacts with the Cohesin Complex to Maintain Chromatid Separation and Gene Expression for Normal Hematopoiesis
BioProject PRJNA327562
SRA SRP077725

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Supplementary file Size Download File type/resource
GSE83960_RAW.tar 862.0 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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