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Series GSE83772 Query DataSets for GSE83772
Status Public on Jun 30, 2017
Title The FAM46C gene encodes a non-canonical poly(A) polymerase and acts as an onco-suppressor in multiple myeloma
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary FAM46C is one of the most frequently mutated genes in multiple myeloma (MM) and encodes a protein of unknown function. Using a combination of in vitro and in vivo approaches, we demonstrate that FAM46C encodes an active cytoplasmic non-canonical poly(A) polymerase, which enhances mRNA stability and gene expression. Moreover, we also found that the reintroduction of active FAM46C into MM cell lines, but not its catalytically-inactive mutant, leads to broad polyadenylation and stabilization of mRNAs strongly enriched with those encoding endoplasmic reticulum-targeted proteins and induced cell death. This is, to our knowledge, the first report that directly associates cytoplasmic poly(A) polymerase with carcinogenesis. Furthermore, our data suggest that the human genome encodes at least eleven non-canonical poly(A) polymerases with four FAM46 family members. Since FAM46 proteins are differentially expressed during development, these proteins may positively regulate transcript stability and translational rate in a tissue-specific manner.
Overall design The H929 and SKMM1 MM cells were transduced with lentiviruses carrying FAM46CWTGFP (WT) or FAM46CD90A,D92AGFP (catalitic mutant). 72h after transgene delivery total RNA was extracted and RNA-seq libraries were prepared.
Contributor(s) Mroczek S, Kulinski TM, Chlebowska J, Nowis D, Dziembowski A
Citation(s) 28931820
Submission date Jun 27, 2016
Last update date May 15, 2019
Contact name Tomasz M. KuliƄski
Phone 225970602
Organization name International Institute of Molecular and Cell Biology in Warsaw
Lab Andrzej Dziembowski
Street address ul. Ks. Trojdena 4
City Warszawa
State/province Mazowieckie
ZIP/Postal code 02-109
Country Poland
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (26)
GSM2218701 H929 cells expressing FAM46CD90A,D92AGFP for 72 hrs rep1_polyA RNA
GSM2218702 H929 cells expressing FAM46CD90A,D92AGFP for 72 hrs rep2_polyA RNA
GSM2218703 H929 cells expressing FAM46CD90A,D92AGFP for 72 hrs rep3_polyA RNA
BioProject PRJNA326952
SRA SRP077288

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GSE83772_RAW.tar 24.7 Gb (http)(custom) TAR (of BW, TXT)
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