GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE83757 Query DataSets for GSE83757
Status Public on Aug 14, 2017
Title NFIA Controls the Brown Fat Gene Program by Co-Localizing with PPARgamma at Cell-Type-Specific Enhancers (chromatin)
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Brown fat dissipates energy as heat and protects against obesity. Here, we identified nuclear factor I-A (NFIA) as a novel transcriptional regulator of brown fat by a genome-wide open chromatin analysis of murine brown and white fat followed by motif analysis of brown-fat-specific open chromatin regions. NFIA and the adipogenic master regulator, PPARgamma, co-localize at the brown-fat-specific enhancers. Moreover, the binding of NFIA precedes and facilitates the binding of PPARgamma, leading to increased chromatin accessibility and active transcription. Introduction of NFIA into myoblasts results in brown adipocyte differentiation. Conversely, the brown fat of NFIA knockout mice displays impaired expression of the brown-fat-specific genes and reciprocal elevation of muscle genes. Finally, expression of NFIA and the brown-fat-specific genes is positively correlated in human brown fat. These results indicate that NFIA is a key transcriptional regulator of brown fat and exerts its effects by co-localizing with PPARg at cell-type-specific enhancers.
Overall design FAIRE-seq analysis of murine BAT, iWAT and eWAT, ChIP-seq analysis in immortalized brown adipocytes and NFIA-expressing C2C12 myoblasts, and ATAC-seq analysis in immortalized brown adipocytes.
Contributor(s) Hiraike Y, Waki H, Nakamura M, Suzuki K, Nakaki R, Yamamoto S, Tsutsumi S, Aburatani H, Yamauchi T, Kadowaki T
Citation(s) 28812581
Submission date Jun 27, 2016
Last update date Jul 25, 2021
Contact name Hironori Waki
Organization name The University of Tokyo
Street address Hongo 7-3-1, Bunkyo-ku
City Tokyo
ZIP/Postal code 1138655
Country Japan
Platforms (3)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
GPL16417 Illumina MiSeq (Mus musculus)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (24)
This SubSeries is part of SuperSeries:
GSE83764 NFIA Controls the Brown Fat Gene Program by Co-Localizing with PPARgamma at Cell-Type-Specific Enhancers
BioProject PRJNA326936
SRA SRP077281

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE83757_ATAC-seq_brown_adipocytes_day6_rep1_2.bigWig 256.2 Mb (ftp)(http) BIGWIG
GSE83757_FAIRE_BAT.bdg.bedgraph.gz 359.6 Mb (ftp)(http) BEDGRAPH
GSE83757_FAIRE_eWAT.bdg.bedgraph.gz 180.9 Mb (ftp)(http) BEDGRAPH
GSE83757_FAIRE_iWAT.bdg.bedgraph.gz 203.2 Mb (ftp)(http) BEDGRAPH
GSE83757_NFIA_ChIP-seq_NFIA-expressing_C2C12_myoblasts_day0.bigWig 608.6 Mb (ftp)(http) BIGWIG
GSE83757_NFIA_ChIP-seq_NFIA-expressing_C2C12_myoblasts_day7.bigWig 626.9 Mb (ftp)(http) BIGWIG
GSE83757_NFI_ChIP-seq_brown_adipocytes_day0.bigWig 273.1 Mb (ftp)(http) BIGWIG
GSE83757_NFI_ChIP-seq_brown_adipocytes_day6.bigWig 198.0 Mb (ftp)(http) BIGWIG
GSE83757_PPARg_ChIP-seq_brown_adipocytes_day0_rep1_2.bigWig 173.7 Mb (ftp)(http) BIGWIG
GSE83757_PPARg_ChIP-seq_brown_adipocytes_day6_rep1_2.bigWig 502.0 Mb (ftp)(http) BIGWIG
GSE83757_RAW.tar 3.3 Gb (http)(custom) TAR (of BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap