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Status |
Public on Mar 08, 2017 |
Title |
Ribosome Profiling analysis of Follicular B (FoB) cells purified from WT, miR-17~92 transgenic and miR-17-92 tKO mice |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We have generated a miR-17~92 transgene and fl/fl allele whose expression can be turned on and off conditionally by Cre recombinase. The mice were crossed to CD19-Cre mice to turn on and off the expression of miR-17~92 specifically in B cells and stimulated LPS/IL-4 for 25.5hr. tKO mice were generated in the miR-106a-363-/-;miR-106b-25-/- background, so that all three homologous cluster of miR-17~92 family miRNAs are deleted in B cells.
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Overall design |
Follicular B(FoB) cells were purified from CD19-Cre;miR-17~92 fl/fl;miR-106a-363;miR-106b~25 mice (tKO1, tKO2, tKO3), WT (WT1, WT2, WT3) and transgenic (TG2, TG2, TG3) mice by MACS depletion of cells positive for CD9, CD43, and CD93 (also known as AA4.1). The purified B cells were stimulated with LPS/IL-4 for 25.5hr in B cell media.
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Contributor(s) |
Jin HY, Xiao C |
Citation(s) |
28241004 |
NIH grant(s) |
Grant ID |
Grant title |
Affiliation |
Name |
R01 AI087634 |
The Role of miR-17~92 in Autoimmune Diseases |
THE SCRIPPS RESEARCH INSTITUTE |
Changchun Xiao |
R01 AI089854 |
Functional Analysis of MicroRNAs in Lymphocyte Development and Immune Tolerance |
THE SCRIPPS RESEARCH INSTITUTE |
Changchun Xiao |
R01 AI121155 |
Regulation of T Follicular Helper Cell Differentiation by MicroRNAs |
THE SCRIPPS RESEARCH INSTITUTE |
Changchun Xiao |
R56 AI110403 |
Regulation of T Follicular Helper Cell Differentiation by MicroRNAs |
THE SCRIPPS RESEARCH INSTITUTE |
Changchun Xiao |
R56 AI121155 |
Regulation of T Follicular Helper Cell Differentiation by MicroRNAs |
THE SCRIPPS RESEARCH INSTITUTE |
Changchun Xiao |
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Submission date |
Jun 23, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Changchun Xiao |
E-mail(s) |
cxiao@scripps.edu
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Phone |
(858) 784-7640
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Organization name |
The Scripps Research Institute
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Department |
Immunology & Microbial Science
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Lab |
Changchun Xiao Lab
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Street address |
10550 North Torrey Pines Road
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City |
La Jolla |
State/province |
California |
ZIP/Postal code |
92037 |
Country |
USA |
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Platforms (1) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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Samples (9)
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Relations |
BioProject |
PRJNA326723 |
SRA |
SRP077043 |