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Series GSE83684 Query DataSets for GSE83684
Status Public on Mar 08, 2017
Title Ribosome Profiling analysis of Follicular B (FoB) cells purified from WT, miR-17~92 transgenic and miR-17-92 tKO mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We have generated a miR-17~92 transgene and fl/fl allele whose expression can be turned on and off conditionally by Cre recombinase. The mice were crossed to CD19-Cre mice to turn on and off the expression of miR-17~92 specifically in B cells and stimulated LPS/IL-4 for 25.5hr. tKO mice were generated in the miR-106a-363-/-;miR-106b-25-/- background, so that all three homologous cluster of miR-17~92 family miRNAs are deleted in B cells.
 
Overall design Follicular B(FoB) cells were purified from CD19-Cre;miR-17~92 fl/fl;miR-106a-363;miR-106b~25 mice (tKO1, tKO2, tKO3), WT (WT1, WT2, WT3) and transgenic (TG2, TG2, TG3) mice by MACS depletion of cells positive for CD9, CD43, and CD93 (also known as AA4.1). The purified B cells were stimulated with LPS/IL-4 for 25.5hr in B cell media.
 
Contributor(s) Jin HY, Xiao C
Citation(s) 28241004
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 AI087634 The Role of miR-17~92 in Autoimmune Diseases THE SCRIPPS RESEARCH INSTITUTE Changchun Xiao
R01 AI089854 Functional Analysis of MicroRNAs in Lymphocyte Development and Immune Tolerance THE SCRIPPS RESEARCH INSTITUTE Changchun Xiao
R01 AI121155 Regulation of T Follicular Helper Cell Differentiation by MicroRNAs THE SCRIPPS RESEARCH INSTITUTE Changchun Xiao
R56 AI110403 Regulation of T Follicular Helper Cell Differentiation by MicroRNAs THE SCRIPPS RESEARCH INSTITUTE Changchun Xiao
R56 AI121155 Regulation of T Follicular Helper Cell Differentiation by MicroRNAs THE SCRIPPS RESEARCH INSTITUTE Changchun Xiao
Submission date Jun 23, 2016
Last update date May 15, 2019
Contact name Changchun Xiao
E-mail(s) cxiao@scripps.edu
Phone (858) 784-7640
Organization name The Scripps Research Institute
Department Immunology & Microbial Science
Lab Changchun Xiao Lab
Street address 10550 North Torrey Pines Road
City La Jolla
State/province California
ZIP/Postal code 92037
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (9)
GSM2212597 Control_1_25.5hr
GSM2212598 Control_2_25.5hr
GSM2212599 Control_3_25.5hr
Relations
BioProject PRJNA326723
SRA SRP077043

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE83684_Xiao_Ribosome_Profiling_FPKM_20151217.xls.gz 879.9 Kb (ftp)(http) XLS
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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