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Series GSE83654 Query DataSets for GSE83654
Status Public on Jul 21, 2016
Title Microarray Analysis of DU 145, LNCaP and PC-3 Prostate Cancer Cell Lines upon Docetaxel treatment
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We measured the effect of docetaxel treatment to three differentially responsive prostate cancer cell lines, LNCaP, DU145 and PC-3, based on a transcriptional time course response by microarray analysis. These cell lines represent both androgen independent (DU145 and PC-3) and androgen sensitive (LNCaP) cells
Overall design Hybridized arrays were scanned with Agilent’s dual laser-based scanner. Feature Extraction software version 10.5 (Agilent Technologies) was used to link a feature to a design file and to determine the relative fluorescence intensity between two samples. Dye swap strategy with alternate cy3 and cy5 labeling on docetaxel treated and control groups over four time points was used to have technical replicates and decrease dye bias.
Web link
Contributor(s) Moritz RL, Huang C, Kusebauch U
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Submission date Jun 23, 2016
Last update date Nov 27, 2018
Contact name Robert Moritz
Organization name Institute for Systems Biology
Street address 401 Terry Ave Nth
City Seattle
State/province Washington
ZIP/Postal code 98109
Country USA
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (24)
GSM2211831 PC3_8h_Treatment 1_v_Control 1
GSM2211832 PC3_8h_Treatment 2_v_Control 2
GSM2211833 PC3_24h_Treatment 1_v_Control 1
BioProject PRJNA326601

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE83654.txt.gz 16.2 Mb (ftp)(http) TXT
GSE83654_RAW.tar 505.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data are available on Series record

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