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Series GSE83580 Query DataSets for GSE83580
Status Public on Jun 21, 2017
Title RNA-sequencing of Fes KO and WT melanomas
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Publication abstract: An incomplete view of the (epi)genetic events that drive melanoma initiation and progression has been a major barrier to rational development of effective therapeutics and prognostic diagnostics for melanoma patients. Recent approaches that integrate human melanoma genomic and transcriptomic data provide unprecedented opportunities to discover oncogenic melanoma drivers. One limitation, however, is that human melanoma genome exhibits a radically altered cytogenetic profile. There is therefore the need for biologically-meaningful approaches to identify and validate lesions that drive melanomagenesis. We combined comparative oncogenomic approaches with mouse modeling to identify new cancer genes/pathways that drive melanoma progression. Spontaneously acquired genetic alterations such as copy-number alterations and specific mutations in mouse tumors of defined genetic origin were identified and used to prioritize relevant lesions from the complex human melanoma genomes. This integrated effort confirmed the importance of several genes and pathways previously implicated in melanoma and identified new putative melanoma tumor suppressor genes. Genetic ablation of one such gene, c-Fes, cooperated with BRafv600E to accelerate melanomagenesis in mice. This comparative oncogenomic approach has therefore helped discover a series of novel melanoma tumor suppressor genes, including c-FES, with prognostic and therapeutic relevance in human melanoma.
Overall design RNA extracted from 5 Tyr::CreERT2/+;BRafV600E/+;Ptenl/l;Fes-/- and 5 Tyr::CreERT2/+;BRafV600E/+;Ptenl/l;Fes+/+ melanomas
Contributor(s) Olvedy M, Tisserand J, Luciani F, Boeckx B, Wouters J, Lopez S, Rambow F, Aibar S, Barra J, Köhler C, Radaelli E, Tartare-Deckert S, van den Oord JJ, Aerts S, Dubreuil P, Lambrechts D, De Sepulveda P, Marine J
Citation(s) 28463229
Submission date Jun 21, 2016
Last update date May 15, 2019
Contact name Sara Aibar
Organization name VIB-KU Leuven
Department Center for Brain & Disease Research
Lab Laboratory of Computational Biology
Street address Herestraat 49
City Leuven
ZIP/Postal code 3001
Country Belgium
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (10)
GSM2209888 Fes KO – repl 1
GSM2209889 Fes KO – repl 2
GSM2209890 Fes WT – repl 1
BioProject PRJNA326413
SRA SRP076866

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Supplementary file Size Download File type/resource
GSE83580_RNAseq_SFnorm_log2_counts.txt.gz 1.3 Mb (ftp)(http) TXT
GSE83580_RNAseq_counts.txt.gz 482.1 Kb (ftp)(http) TXT
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