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Series GSE83422 Query DataSets for GSE83422
Status Public on Apr 24, 2017
Title Methyl-seq and ChIP-seq analyses of TSPAN8-high and TSPAN8-low subpopulations of ID4-EGFP+ undifferentiated spermatogonia
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Summary P6 ID4-EGFP+ undifferentiated spermatogonia stained robustly (high) or weakly (low) for TSPAN8 were isolated by FACS.
Overall design Pooled independent sorts of each population were used for reduced-representation methyl-seq or ChIP-seq for three histone modifications (H3K4me3, H3K27Ac and H3K27me3).
Contributor(s) Mutoji K, Cheng K, McCarrey J, Hermann B
Citation(s) 27733379
Submission date Jun 16, 2016
Last update date May 15, 2019
Contact name Brian P. Hermann
Phone 210-458-8047
Organization name University of Texas at San Antonio
Department Department of Biology
Street address 1 UTSA Circle
City San Antonio
State/province TX
ZIP/Postal code 78249
Country USA
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (10)
GSM2202691 TSPAN8-high-Me
GSM2202692 TSPAN8-low-Me
GSM2202693 TSPAN8_High_H3K27Ac_ChIP-seq
BioProject PRJNA325864
SRA SRP076654

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Supplementary file Size Download File type/resource
GSE83422_CpG_result_table.txt.gz 70.7 Mb (ftp)(http) TXT
GSE83422_TSPAN8_ChIP_intervals.xlsx 2.4 Mb (ftp)(http) XLSX
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