|
| Status |
Public on Apr 24, 2017 |
| Title |
Methyl-seq and ChIP-seq analyses of TSPAN8-high and TSPAN8-low subpopulations of ID4-EGFP+ undifferentiated spermatogonia |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
|
| Summary |
P6 ID4-EGFP+ undifferentiated spermatogonia stained robustly (high) or weakly (low) for TSPAN8 were isolated by FACS.
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| |
|
| Overall design |
Pooled independent sorts of each population were used for reduced-representation methyl-seq or ChIP-seq for three histone modifications (H3K4me3, H3K27Ac and H3K27me3).
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| |
|
| Contributor(s) |
Mutoji K, Cheng K, McCarrey J, Hermann B |
| Citation(s) |
27733379 |
| Submission date |
Jun 16, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Brian P. Hermann |
| E-mail(s) |
Brian.Hermann@utsa.edu
|
| Phone |
210-458-8047
|
| Organization name |
University of Texas at San Antonio
|
| Department |
Department of Biology
|
| Street address |
1 UTSA Circle
|
| City |
San Antonio |
| State/province |
TX |
| ZIP/Postal code |
78249 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (10)
|
|
| Relations |
| BioProject |
PRJNA325864 |
| SRA |
SRP076654 |
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