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Series GSE83295 Query DataSets for GSE83295
Status Public on Dec 28, 2017
Title AP-1 transcription factors and the BAF complex mediate signal-dependent enhancer selection
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Enhancer elements are regulatory sequences within genomes that direct the selective expression of genes so that genetically identical cells can differentiate and acquire the highly specialized forms and functions required to build a functioning animal. To differentiate, cells must select from among the ~10^6 enhancers encoded in the genome the thousands of enhancers that drive the gene programs that impart their distinct features. We used a genetic approach to identify transcription factors (TFs) required for enhancer selection in fibroblasts. This revealed that the broadly expressed, growth factor-inducible TFs Fos/Jun (AP-1) play a central role in enhancer selection. Fos/Jun selects enhancers together with cell type-specific TFs by collaboratively binding to nucleosomal enhancers and directing recruitment of the SWI/SNF (BAF) chromatin remodeling complex to establish accessible chromatin. These experiments demonstrate how environmental signals acting via Fos/Jun and BAF coordinate with cell type-specific TFs to select enhancer repertoires that enable differentiation during development.
Overall design Genome-wide maps of H3K27ac, H3K4me1/2, AP-1 transcription factors, Smarca4, and chromatin accessibility before and after serum stimulation for the indicated times in primary mouse embryonic fibroblasts.
Contributor(s) Ling E, Vierbuchen T, Greenberg ME
Citation(s) 29272704
Submission date Jun 13, 2016
Last update date May 15, 2019
Contact name Michael Greenberg
Organization name Harvard Medical School
Street address 200 Longwood Ave
City Boston
ZIP/Postal code 02215
Country USA
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (107)
GSM2198456 CHX H3K27ac 0hr B1
GSM2198457 CHX H3K27ac 0hr B2
GSM2198458 CHX H3K27ac 90m B1
BioProject PRJNA325508
SRA SRP076482

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Supplementary file Size Download File type/resource
GSE83295_RAW.tar 22.2 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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