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Series GSE83228 Query DataSets for GSE83228
Status Public on Oct 06, 2016
Title Signal-Dependent Recruitment of BRD4 to Cardiomyocyte Super-Enhancers is Suppressed by a MicroRNA [RNA-Seq]
Organism Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary BRD4 governs pathological cardiac gene expression by binding acetylated chromatin, resulting in enhanced RNA polymerase II (Pol II) phosphorylation and transcription elongation. Here, we describe a signal-dependent mechanism for regulation of BRD4 in cardiomyocytes. BRD4 expression is suppressed by microRNA-9 (miR-9), which targets the 3’ untranslated region of the Brd4 transcript. In response to stress stimuli, miR-9 is downregulated, leading to derepression of BRD4 and enrichment of BRD4 at long-range super-enhancers (SEs) associated with pathological cardiac genes. A miR-9 mimic represses stimulus-dependent targeting of BRD4 to SEs and blunts Pol II phosphorylation at proximal transcription start sites, without affecting BRD4 binding to SEs that control constitutively expressed cardiac genes. These findings suggest that dynamic enrichment of BRD4 at SEs genome-wide serves a crucial role in the control of stress-induced cardiac gene expression, and define a miR-dependent signaling mechanism for the regulation of chromatin state and Pol II phosphorylation.
Overall design RNA-seq in neonatal rat ventricular myocytes
Contributor(s) McKinsey TA, Stratton MS, Halder SM, Lin CC, Bradner JE
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NIH grant(s)
Grant ID Grant title Affiliation Name
R01 HL127240 Regulation of Chromatin Signaling in Heart Failure by BET Bromodomain Proteins UNIVERSITY OF COLORADO DENVER McKinsey
F32 HL126354 Role of BET proteins in cardiac remodeling UNIVERSITY OF COLORADO DENVER Stratton
Submission date Jun 10, 2016
Last update date May 15, 2019
Contact name Timothy McKinsey
Phone 303-724-5479
Organization name University of Colorado Anschutz Medical Campus
Department Division of Cardiology
Lab McKinsey
Street address 12700 E. 19th Ave (8450E)
City Aurora
State/province CO
ZIP/Postal code 80045
Country USA
Platforms (1)
GPL14844 Illumina HiSeq 2000 (Rattus norvegicus)
Samples (8)
GSM2196864 PE_3351
GSM2196865 PE_3352
GSM2196866 PE_mir9_3354
This SubSeries is part of SuperSeries:
GSE83230 Signal-Dependent Recruitment of BRD4 to Cardiomyocyte Super-Enhancers is Suppressed by a MicroRNA
BioProject PRJNA325308
SRA SRP076389

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Supplementary file Size Download File type/resource
GSE83228_gene_expression.xlsx.gz 1.3 Mb (ftp)(http) XLSX
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Raw data are available in SRA

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