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Series GSE83145 Query DataSets for GSE83145
Status Public on Oct 31, 2017
Title RNA profiling of testis from wild-type and tamoxifen-induced NIPP1 knockout mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary This study aimed to explore the role of NIPP1 in adult germline cell proliferation and differentiation, using a ubiquitous inducible NIPP1 knockout (TKO) mouse model. To gain unbiased insight into the molecular mechanism that underly the sertoli-only phenotype in TKO, we performed a comparative RNA sequencing profiling of control and TKO, in which NIPP1 was tamoxifin-induced depleted.
Overall design Two genotypes are compared after treatment with tamoxifen. The control genotype (UBC CRE-ERT2+/- Ppp1r8 fl/+) looses the floxed allele of PPP1R8 (aka NIPP1) as a consequence of the treatment with tamoxifen and becomes heterozygous for PPP1R8. The KO genotype (UBC CRE-ERT2+/- Ppp1r8 fl/-) also looses the floxed allele of PPP1R8 as a consequence of the tamoxifen treatment and becomes homozygous KO. For each genotype, 4 replicates are profiled.
Contributor(s) Ferreira M, Boens S, Szekér K, Verbinnen I, Van Eynde A, Fardilha M, Bollen M
Citation(s) 29042623
Submission date Jun 09, 2016
Last update date May 15, 2019
Contact name Rekin's Janky
Organization name VIB
Department Nucleomics Core
Street address Herestraat 49 Box 816
City Leuven
ZIP/Postal code B-3000
Country Belgium
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (8)
GSM2195195 NIPP1_CTR_testis_mouse1
GSM2195196 NIPP1_CTR_testis_mouse2
GSM2195197 NIPP1_CTR_testis_mouse3
BioProject PRJNA325121
SRA SRP076338

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Supplementary file Size Download File type/resource
GSE83145_RNAseqCounts.txt.gz 372.3 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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