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Series GSE83126 Query DataSets for GSE83126
Status Public on Feb 03, 2017
Title Effect of 48h treatment with 100nM GSK2879552 on T-ALL cell lines LOUCY and PEER
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary RNA sequencing was performed on biological triplicates of LOUCY and PEER cells treated with 100nM GSK2879552 for 48h versus their corresponding DMSO treatment controls. GSK2879552 is a potent, selective and orally bioavailable inhibitor of the lysine-specific histone demethylase 1 (LSD1). Gene expression was severely affected by LSD1 inhibition. Pathway analysis performed on the differentially expressed genes following 48hrs of LSD1i treatment clearly showed an increased apoptotic gene signature (CASP3, CASP8) and enhanced expression of genes associated with TRAIL signaling. Furthermore, pre-ranked Gene Set Enrichment Analysis (GSEA) revealed that genes upregulated after LSD1 inhibition in LOUCY showed significant enrichment for transcripts that are down in hematopoietic stem cells and early T lymphocytes. In contrast, important components of the Notch, WNT and SMAD signalling complex were significantly down regulated upon short-term LSD1i treatment in LOUCY cells. In line with this notion, pre-ranked GSEA also revealed that LSD1i resulted in decreased expression of genes that significantly overlap with an oncogenic MYC signature.
Overall design LOUCY and PEER cells were treated with 100nM GSK2879552 or DMSO for 48h. Three biological replicates of this treatment were performed for each celline.
Contributor(s) Peirs S, Goossens S, Van Loocke W, Van Vlierberghe P, Haigh J
Citation(s) 28069602
Submission date Jun 08, 2016
Last update date May 15, 2019
Contact name Sofie Peirs
Organization name Ghent University
Street address De Pintelaan 185
City Gent
ZIP/Postal code 9000
Country Belgium
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (12)
GSM2193306 LOUCY_DMSO_48h_rep1
GSM2193307 LOUCY_100nMGSK2879552_48h_rep1
GSM2193308 LOUCY_DMSO_48h_rep2
This SubSeries is part of SuperSeries:
GSE83128 Oncogenic ZEB2 activation drives sensitivity towards LSD1 inhibition in T-cell acute lymphoblastic leukemia
BioProject PRJNA324827
SRA SRP076286

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Supplementary file Size Download File type/resource
GSE83126_RAW.tar 4.3 Mb (http)(custom) TAR (of TAB)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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