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Series GSE83094 Query DataSets for GSE83094
Status Public on Oct 05, 2016
Title RBYP stimulates PRC1 to shape chromatin-based communication between polycomb repressive complexes [ChIP-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Polycomb Repressive Complexes (PRC), and their chromatin-modifying activities, are essential for the correct regulation of gene expression during cellular differentiation and development. Although their role in transcriptional repression is well described, a detailed molecular understanding of their complex assembly and enzymatic activity has been lacking. We therefore set out to characterize the relationship between PRC1 complex composition and its ability to catalyse H2AK119ub1 for one of the most abundant PRC1 complexes in embryonic stem cells, PCGF1-PRC1. Biochemical reconstitution of the PCGF1-PRC1 complex revealed that RYBP/YAF2 was essential for robust enzymatic activity of the complex. Using calibrated ChIP-seq for H2AK119ub1, we identify that RYBP-dependent PRC1 activity has a widespread role in shaping H2AK119ub1 levels genome-wide in mouse embryonic stem cells. Furthermore, these H2AK119ub1 levels stimulate PRC2 activity as part of an activity-based feedback loop, which we demonstrate is required for maintenance of transcriptional repression. Together, these observations uncover complex-based principles for PRC1 assembly and activity, and further our understanding of Polycomb domain function.
Overall design RYBPfl/fl;YAF2-/- ES cells were treated with tamoxifen (OHT) for 96 hours prior to ChIP-seq for RYBP, RING and SUZ12.
Contributor(s) Rose NR, Blackledge NP, King HW, Klose RJ
Citation(s) 27705745
Submission date Jun 07, 2016
Last update date May 15, 2019
Contact name Hamish W King
Organization name Queen Mary University of London
Department Blizard Institute
Street address 4 Newark St
City London
ZIP/Postal code E1 2AT
Country United Kingdom
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (24)
GSM2192980 RYBPfl/fl.YAF2KO_RYBP_UNT_rep1
GSM2192981 RYBPfl/fl.YAF2KO_RYBP_UNT_rep2
GSM2192982 RYBPfl/fl.YAF2KO_RYBP_UNT_rep3
This SubSeries is part of SuperSeries:
GSE83135 RBYP stimulates PRC1 to shape chromatin-based communication between polycomb repressive complexes
BioProject PRJNA325003
SRA SRP076304

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Supplementary file Size Download File type/resource 210.1 Mb (ftp)(http) BW 210.4 Mb (ftp)(http) BW 525.8 Mb (ftp)(http) BW 538.4 Mb (ftp)(http) BW 512.6 Mb (ftp)(http) BW 510.7 Mb (ftp)(http) BW 471.9 Mb (ftp)(http) BW 478.5 Mb (ftp)(http) BW
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