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| Status |
Public on Jun 20, 2008 |
| Title |
HEK293 cells overexpressing HNF4a2 wild type or mutant forms C106R or R154X |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Identification of genes regulated by the transcription factor HNF4a2
Keywords: ordered
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| |
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| Overall design |
We used microarrays to identify genes regulated by the transcription factor HNF4a2. HEK293 cell lines containing doxycyclin-inducible wilt type or mutant forms of HNF4a2m which were introduced by FRT/FLP mediated recombination into FLP-In T-REx 293 cells (Invitrogen) were treated for 24 hours with 1 µg/ml doxycyclin. Gene expression profiles of induced and noninduced cells were compared to identify HNF4 regulated targets.
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| |
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| Contributor(s) |
Grigo K, Wirsing A, Lucas B, Klein-Hitpass L, Ryffel GU |
| Citation(s) |
16007190, 18163890 |
| Submission date |
Jun 26, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Ludger Klein-Hitpass |
| E-mail(s) |
ludger.klein-hitpass@uni-essen.de
|
| Phone |
+49 201 723 85552
|
| Organization name |
Institut fuer Zellbiologie
|
| Department |
Universitaetsklinikum
|
| Lab |
BioChip Lab
|
| Street address |
Virchowstr. 173
|
| City |
Essen |
| ZIP/Postal code |
D-45122 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (8)
|
| GSM205753 |
HEK293 cell line #1 with noninduced HNF4a2 wild type |
| GSM205754 |
HEK293 cell line #1 with induced HNF4a2 wild type |
| GSM205755 |
HEK293 cell line #4 with noninduced HNF4a2 wild type |
| GSM205756 |
HEK293 cell line #4 with induced HNF4a2 wild type |
| GSM205757 |
HEK293 cell line #1 with noninduced HNF4a2 mutant C106R |
| GSM205758 |
HEK293 cell line #1 with induced HNF4a2 mutant C106R |
| GSM205759 |
HEK293 cell line #6 with noninduced HNF4a2 mutant R154X |
| GSM205760 |
HEK293 cell line #6 with induced HNF4a2 mutant R154X |
|
| Relations |
| BioProject |
PRJNA101255 |
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