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Series GSE82243 Query DataSets for GSE82243
Status Public on Oct 06, 2016
Title Signal-Dependent Recruitment of BRD4 to Cardiomyocyte Super-Enhancers is Suppressed by a MicroRNA [ChIP-Seq]
Organism Rattus norvegicus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary BRD4 governs pathological cardiac gene expression by binding acetylated chromatin, resulting in enhanced RNA polymerase II (Pol II) phosphorylation and transcription elongation. Here, we describe a signal-dependent mechanism for regulation of BRD4 in cardiomyocytes. BRD4 expression is suppressed by microRNA-9 (miR-9), which targets the 3’ untranslated region of the Brd4 transcript. In response to stress stimuli, miR-9 is downregulated, leading to derepression of BRD4 and enrichment of BRD4 at long-range super-enhancers (SEs) associated with pathological cardiac genes. A miR-9 mimic represses stimulus-dependent targeting of BRD4 to SEs and blunts Pol II phosphorylation at proximal transcription start sites, without affecting BRD4 binding to SEs that control constitutively expressed cardiac genes. These findings suggest that dynamic enrichment of BRD4 at SEs genome-wide serves a crucial role in the control of stress-induced cardiac gene expression, and define a miR-dependent signaling mechanism for the regulation of chromatin state and Pol II phosphorylation.
 
Overall design ChIP-Seq in neonatal rat ventricular myocytes
 
Contributor(s) McKinsey TA, Stratton MS, Lin CY, Haldar SM, Bradner JE
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NIH grant(s)
Grant ID Grant title Affiliation Name
R01 HL127240 Regulation of Chromatin Signaling in Heart Failure by BET Bromodomain Proteins UNIVERSITY OF COLORADO DENVER McKinsey
F32 HL126354 Role of BET proteins in cardiac remodeling UNIVERSITY OF COLORADO DENVER Stratton
Submission date Jun 03, 2016
Last update date May 15, 2019
Contact name Timothy McKinsey
E-mail(s) TIMOTHY.MCKINSEY@UCDENVER.EDU
Phone 303-724-5479
Organization name University of Colorado Anschutz Medical Campus
Department Division of Cardiology
Lab McKinsey
Street address 12700 E. 19th Ave (8450E)
City Aurora
State/province CO
ZIP/Postal code 80045
Country USA
 
Platforms (1)
GPL14844 Illumina HiSeq 2000 (Rattus norvegicus)
Samples (8)
GSM2187051 NRVM_BRD4_R1
GSM2187052 NRVM_PE_BRD4_R1
GSM2187053 NRVM_PE_MIR9MIMIC_BRD4_R1
This SubSeries is part of SuperSeries:
GSE83230 Signal-Dependent Recruitment of BRD4 to Cardiomyocyte Super-Enhancers is Suppressed by a MicroRNA
Relations
BioProject PRJNA324424
SRA SRP076108

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE82243_RAW.tar 593.6 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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