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Series GSE82147 Query DataSets for GSE82147
Status Public on Jun 01, 2017
Title Genome wide impact of loss of mechanoluminal stimulation on neonatal intestine
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: Surgical treatment of congenital and neonatal intestinal diseases often requires resection of intestine and/or diverstion of luminal flow. Subsequent growth on enteral nutrition alone depends on adaptation of the remaining bowel. The role of mechanoluminal stimulation in driving intestinal adaptation and the effects of depriving the intestine of mechanoluminal stimulation are unknown.
Methods: 5 pairs of intestine from neonatal surgical patients were obtained at ileostomy reversal. The proximal segment contiguous with enteral feeding was denoted “fed” and the distal segment without luminal flow was denoted “unfed". Following RNA extraction, these samples underwent deep sequencing.
Results: Fed intestine had increased expression of genes involved in inflammation and immune regulation and steroid secretion. Unfed intestine had increased expression of genes involved in digestion and transport.
Conclusion: Independent of external influences, the presence or absence of enteral mechanoluminal stimulation causes significant alterations in gene expression in intestine.
Overall design Profiles of paired fed and unfed neonatal intestine were generated by deep sequencing in triplicate.
Contributor(s) Wieck M, Grikscheit T, Grubbs B, Thornton M
Citation(s) 28462379
Submission date Jun 02, 2016
Last update date May 15, 2019
Contact name Tracy Grikscheit
Organization name Children's Hospital Los Angeles
Street address 4650 Sunset Boulevard Mailstop#100
City Los Angeles
State/province California
ZIP/Postal code 90027
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (10)
GSM2184758 117a
GSM2184759 117b
GSM2184760 136a
BioProject PRJNA324176
SRA SRP076010

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE82147_01Jun16_fed_unfed_DE_annotated_RPKM.txt.gz 3.1 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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