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Series GSE82104 Query DataSets for GSE82104
Status Public on Mar 08, 2017
Title Acriflavine inhibits the epithelial-to-mesenchymal transition in vitro in liver and pancreatic cancer cells (part of study on PANC1 cells treated with CoCl2)
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Background: Epithelial-to-mesenchymal transition (EMT) is considered an important driving mechanism behind aggressive cancer phenotype. This was recently challenged by the finding that cells can metastasize without undergoing EMT. However, the same studies confirmed the important role of the EMT program in drug resistance. The EMT program is largely dependent on the cell’s microenvironment. Acriflavine (ACF) is a heteroaromatic dye with antibacterial and antiviral effects. Recently, ACF was suggested as anticancer agent for its topoisomerase inhibitor activity. ACF further blocks the hypoxia-inducible factor (HIF) pathway, an important driver of cancer aggressiveness. How ACF works in cancer is however unknown.
Aim: Identification of the working mechanism, molecular pathways and signaling of ACF in EMT cancer cells. To this end, three in vitro models were developed of EMT induction (human pancreatic cancer cells stimulated with TGF-b1, human pancreatic cancer cells stimulated with CoCl2, drug resistance against sorafenib in human liver cancer cells). Only the second model - PANC1 with CoCl2, a model of severe hypoxia - is discussed in this GEO submission.
Overall design Three conditions were investigated: (1) untreated PANC1 cells (= controls), (2) PANC1 cells treated with only CoCl2, (3) PANC1 cells treated with CoCl2 and ACF. For each condition three biological replicates were profiled.
Contributor(s) Dekervel J, Bulle A, Windmolders P, Lambrechts D, Van Cutsem E, Verslype C, van Pelt J
Citation(s) 27987431
Submission date Jun 01, 2016
Last update date May 15, 2019
Contact name Rekin's Janky
Organization name VIB
Department Nucleomics Core
Street address Herestraat 49 Box 816
City Leuven
ZIP/Postal code B-3000
Country Belgium
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (9)
GSM2183482 PANC1_Control1
GSM2183483 PANC1_Control2
GSM2183484 PANC1_Control3
This SubSeries is part of SuperSeries:
GSE82299 Acriflavine inhibits the epithelial-to-mesenchymal transition in vitro in liver and pancreatic cancer cells
BioProject PRJNA324011
SRA SRP075959

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Supplementary file Size Download File type/resource
GSE82104_RNAseqCounts.txt.gz 497.4 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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