Other Expression profiling by high throughput sequencing
Summary
We propose a technology B-HIVE, which allows us to map HIV integrations in a cell population and measure their individual transcription. The principle of B-HIVE is to tag individual viral genomes with a unique barcode of 20 nucleotides that allows us to track the viral transcripts produced by each provirus in the cell population.
Overall design
We performed two independent infection on Jurkat T cells by barcoded HIV (2 biological replicates). Each sample preparation and data analysis was done in parallel in 2 replicates.
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