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Series GSE82061 Query DataSets for GSE82061
Status Public on Sep 17, 2016
Title Position effects influence HIV provirus latency reversal
Organism Homo sapiens
Experiment type Other
Expression profiling by high throughput sequencing
Summary We propose a technology B-HIVE, which allows us to map HIV integrations in a cell population and measure their individual transcription. The principle of B-HIVE is to tag individual viral genomes with a unique barcode of 20 nucleotides that allows us to track the viral transcripts produced by each provirus in the cell population.
Overall design We performed two independent infection on Jurkat T cells by barcoded HIV (2 biological replicates). Each sample preparation and data analysis was done in parallel in 2 replicates.
Contributor(s) Chen H, Zorita E, Filion G
Citation(s) 27870832
Submission date May 31, 2016
Last update date May 15, 2019
Contact name Guillaume Filion
Organization name CRG
Department GRSCC
Lab Genome Architecture
Street address Dr. Aiguader 88
City Barcelona
ZIP/Postal code 08003
Country Spain
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (60)
GSM2182754 Rep1_integration_map
GSM2182755 Rep2_integration_map
GSM2182756 Rep1_barcode_PCR1
BioProject PRJNA323993
SRA SRP075951

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE82061_Jurkat_BHIVE_mini_drug.txt.gz 612.5 Kb (ftp)(http) TXT
GSE82061_Jurkat_BHIVE_mini_expr.txt.gz 126.7 Kb (ftp)(http) TXT
GSE82061_Jurkat_BHIVE_mini_integ.txt.gz 125.6 Kb (ftp)(http) TXT
GSE82061_Jurkat_BHIVE_mini_latent.txt.gz 105.9 Kb (ftp)(http) TXT
GSE82061_Jurkat_WT_mRNAseq.txt.gz 1.2 Mb (ftp)(http) TXT
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Processed data are available on Series record
Raw data are available in SRA

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