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Series GSE82032 Query DataSets for GSE82032
Status Public on May 30, 2017
Title Human basal-like breast cancer cell line HCC1143 treated with BET inhibitor JQ1 in combination with MEK inhibitor Trametinib or PI3K/mTOR inhibitor BEZ235
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The goal of this experiment was to understand the changes in gene expression in the human basal-like breast cancer cell line HCC1143 following treatment with the MEK inhibitor Trametinib (T), PI3K/mTOR inhibitor BEZ235 (B), the BET inhibition JQ1 (JQ), Trametinib + JQ1 (TJ), or BEZ235 + JQ1(BJ), compared to a DMSO control (D). Samples were treated for 72hr and run in triplicate.
Overall design The human basal-like breast cancer cell line HCC1143 was treated for 72hr with 1uM Trametinib (T), 1uM BEZ235, 1uM JQ1 (JQ), 1uM Trametinib + 1uM JQ1 (TJ), 1uM BEZ235 + 1uM JQ1 (BJ), or a 0.05% DMSO control. Total RNA was isolated using a QIAGEN total RNA RNeasy kit, libraries were generated with a Truseq kit, and samples were run on the Nextseq500, data processing is described below.
Contributor(s) Risom T, Sears RC
Citation(s) 30232459
Submission date May 30, 2016
Last update date Jul 25, 2021
Contact name Tyler Risom
Organization name Oregon Health & Science University
Department Molecular Medical Genetics
Lab Dr. Rosalie Sears
Street address 2730 SW Moody Ave
City Portland
State/province Oregon
ZIP/Postal code 97201
Country USA
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (21)
GSM2181588 HCC1143_DMSO
GSM2181589 HCC1143_Trametinib
GSM2181590 HCC1143_BEZ235
BioProject PRJNA323723
SRA SRP075882

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE82032_RAW.tar 29.1 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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