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Series GSE82024 Query DataSets for GSE82024
Status Public on May 31, 2018
Title Expression data from human T24 cells transfected with siNC or siNF90
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary NF90 is a double-stranded RNA binding protein that takes part in microRNA biogenesis, as a competitor for the association of the microprocessor complex with pri-miRNAs, leading to a decrease in miRNA production. We want to know which miRNAs changed in human bladder cancer cell T24 when we downregulated NF90.
We used microarrays to detail the mature miRNA expression and identified distinct classes of 2-fold changed miRNAs during this process.
Overall design T24 cells were transfected with control siRNA (siNC) and NF90 siRNA (siNF90). After verifying the knockdown efficiency, cells were used for RNA extraction and hybridization on Affymetrix microarrays.
Contributor(s) Zhuang J, Yan J
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Submission date May 30, 2016
Last update date Jun 01, 2018
Contact name Junlong Zhuang
Organization name Institute of Urology, Drum Tower hospital
Street address 321# Zhongshan Road
City Nanjing
ZIP/Postal code 210008
Country China
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (4)
GSM2181546 T24 cells_control siRNA_rep1
GSM2181547 T24 cells_control siRNA_rep2
GSM2181548 T24 cells_NF90 SiRNA_rep1
BioProject PRJNA324087

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Supplementary file Size Download File type/resource
GSE82024_RAW.tar 2.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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