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Series GSE81975 Query DataSets for GSE81975
Status Public on Nov 15, 2016
Title Gene expression profile of regenerated CD8αα T cells and CD8αβ T cells from LMP2 T-iPSCs
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Whereas cytotoxic T lymphocytes (CTLs) represent the most promising therapeutic avenue in cancer immunotherapy, adaptive transfer of antigen-specific CTLs has faced difficulty in efficient expansion of CTLs from patients in ex vivo culture. To solve this issue, several groups have proposed that the induced pluripotent stem cell (iPSC) technology can be applied for the expansion of antigen-specific CTLs: when iPSCs are produced from antigen-specific CTLs and CTLs are induced from these iPSCs, all regenerated CTLs express the same TCR as original CTLs. However, in these previous studies, one critical issue remains to be solved. With current methods, the regenerated CTLs are mostly of the CD8αα+ innate type and have less antigen-specific cytotoxic activity than primary CTLs. Here we report that, by stimulating purified iPSC-derived CD4/CD8 double positive (DP) cells with anti-CD3 antibody, T cells expressing CD8αβ were generated and they had cytotoxic activity comparable to primary CTLs.
Overall design mRNA profiles of regenerated CD8αα T cells and CD8αβ T cells from LMP2 T-iPSCs and CD8αα T cells, CD8αβ T cells and γδ T cells from peripheral blood were generated by deep sequencing, in triplicate, using Illumina HiSeq 3000/HiSeq 4000.
Contributor(s) Maeda T, Kataoka K, Kawamoto H
Citation(s) 27872100
Submission date May 27, 2016
Last update date May 15, 2019
Contact name Takuya Maeda
Phone 81-75-751-3818
Organization name Institute for Frontier Medical Science, Kyoto University
Department Department of Immunology
Street address 53 Kawahara-cho Shogoin Sakyo-ku
City Kyoto
State/province Kyoto
ZIP/Postal code 606-8507
Country Japan
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (15)
GSM2178975 LMP2_CD8aa_rep1
GSM2178976 LMP2_CD8aa_rep2
GSM2178977 LMP2_CD8aa_rep3
BioProject PRJNA323549
SRA SRP075805

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Supplementary file Size Download File type/resource
GSE81975_LMP2_RNAseq_exp.txt.gz 2.1 Mb (ftp)(http) TXT
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