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Series GSE81951 Query DataSets for GSE81951
Status Public on Feb 13, 2018
Title Evaluation of the immunogenicity of live-attenuated influenza vaccines in nasal epithelial cells in primary differentiated human nasal epithelial cells [RNA-Seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The host innate immune response to influenza virus is a key determinant of pathogenic outcomes and long-term protective responses against subsequent exposures. Comparison of the transcriptional profiles obtained 24 and 36 hrs post-infection showed that the magnitude of gene expression was greater in LAIV infected cells relative to that observed in WT infected cells. Functional enrichment analysis revealed that the antiviral and inflammatory responses was largely driven by type III IFN induction in both WT and LAIV infected cells. However, the enrichment of biological pathways involved in the recruitment of mononuclear leukocytes, antigen presenting cells, and T lymphocytes was uniquely observed in LAIV infected cells. These findings indicate that cell-intrinsic type III IFN-mediated innate immune responses in the nasal epithelium are not only crucial for viral clearance and attenuation, but may also play an important role in the immunogenicity of live-attenuated vaccines
Overall design Differentiated hNECs were infected at a multiplicity of infection (MOI) of 5 50% tissue culture infectious dose per cell. Prior to infection, cells were apically washed and the basolateral media was refreshed with 500 μl of fresh LHC Basal Medium. The virus inoculum was added to the apical compartment and incubated for 2 hours, after which the inoculum was aspirated and the apical chamber was washed three times. The plates were then returned to the incubator. RNA was isolated from Trizol homogenates from samples harvested at 24hpi and 36hpi. Gene expression microarray analysis was conducted on these samples.
Contributor(s) Forero A, Fenstermacher K, Wohlgemuth N, Carter V, Smith E, Nishida A, Peng X, Hayes M, Francis D, Treanor J, Morrison J, Klein SL, Lane A, Katze M, Pekosz A
Citation(s) 28967519
Submission date May 26, 2016
Last update date May 15, 2019
Contact name Michael Katze
Organization name University of Washington
Department Microbiology
Lab Michael G. Katze, Ph.D
Street address Rosen Building 960 Republican St.
City Seattle
State/province WA
ZIP/Postal code 98109-4325
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (6)
GSM2178351 FLU.H.2014.01.54FUR0056.0d.RNA.Access_S18
GSM2178352 FLU.H.2014.01.54FUR0056.7d.RNA.Access_S23
GSM2178353 FLU.H.2014.01.54FUR0065.3d
This SubSeries is part of SuperSeries:
GSE83285 Evaluation of the immunogenicity of live-attenuated influenza vaccines in nasal epithelial cells in primary differentiated human nasal epithelial cells
BioProject PRJNA323488
SRA SRP075770

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE81951_URnasal_matrix_ps1.txt.gz 256.8 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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