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Series GSE81849 Query DataSets for GSE81849
Status Public on Jun 14, 2016
Title mRNA cap methylation in pluripotency and differentiation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The mRNA cap recruits factors essential for transcript processing and translation initiation.  We report that regulated mRNA cap methylation is a feature of embryonic stem cell (ESC) differentiation.  Expression of the mRNA cap methyltransferase activating subunit, RAM, is elevated in ESCs resulting in high levels of mRNA cap methylation and expression of Oct4 and Sox2 and other pluripotency-associated factors.  During neural differentiation, RAM is suppressed which is required for loss of Oct4 and Sox2 and correct expression of neural markers.
Overall design Cells were treated with control or RAM siRNA and cytosolic RNA or polysome RNA (actively translated) was sequenced.
Contributor(s) Suska O, Cowling V
Citation(s) 27452456
Submission date May 24, 2016
Last update date May 15, 2019
Contact name Victoria Cowling
Organization name University of Dundee
Department School of Life Sciences
Lab Cowling lab
Street address Dow Street
City Dundee
ZIP/Postal code DD1 5EH
Country United Kingdom
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (16)
GSM2176662 input Ct rep1
GSM2176663 input Ct rep2
GSM2176664 input Ct rep3
BioProject PRJNA322696
SRA SRP075667

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Supplementary file Size Download File type/resource
GSE81849_ESC_siRAM_counts.txt.gz 663.9 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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