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Series GSE81840 Query DataSets for GSE81840
Status Public on Feb 17, 2017
Title SIRT7 is an RNA-activated protein lysine deacylase [RIP-seq ribo-zero]
Organism Homo sapiens
Experiment type Other
Summary We report that RNA can increase the catalytic efficiency of SIRT7 and that SIRT7 can remove long chain fatty acyl groups more efficiently than removing acetyl groups. Truncation and mutagenesis studies revealed residues at both the amino and carboxyl termini of SIRT7 that are involved in RNA-binding and important for activity. RNA immunoprecipitation-sequencing (RIP-seq) identified ribosomal RNA (rRNA) as the predominant RNA binding partners of SIRT7. The associated RNA was able to effectively activate the deacetylase and defatty-acylase activities of SIRT7. These findings provide important insights into the biological functions of SIRT7, as well as an improved platform to develop SIRT7 modulators.
Overall design SIRT7 with a Flag tag was expressed in HEK293T cells. Anti-Flag antibody (Sigma #A2220) was used to pull down SIRT7 along with the RNA that were bound to SIRT7. The RNA associated with SIRT7 was examined using next-generation sequencing.
Contributor(s) Tong Z, Grenier JK, Lin H
Citation(s) 27997115
Submission date May 24, 2016
Last update date May 15, 2019
Contact name Jennifer K Grenier
Organization name Cornell University
Department Biomedical Sciences
Lab Biotechnology Building rm 333
Street address 526 Campus Rd
City Ithaca
State/province NY
ZIP/Postal code 14853
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (4)
GSM2176506 total input RNA, ribo-zero rep1
GSM2176507 total input RNA, ribo-zero rep2
GSM2176508 SIRT7 RIP RNA, ribo-zero rep1
This SubSeries is part of SuperSeries:
GSE81841 SIRT7 is an RNA-activated protein lysine deacylase
BioProject PRJNA322677
SRA SRP075660

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Supplementary file Size Download File type/resource
GSE81840_gene_exp.diff.gz 779.8 Kb (ftp)(http) DIFF
GSE81840_genes.read_group_tracking.txt.gz 1.0 Mb (ftp)(http) TXT
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