 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 24, 2018 |
| Title |
Maintenance of Mammalian Stem Cell States by Honeybee Royalactin |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Royal Jelly (RJ) is the queen-maker for the honeybee Apis mellifera, and has documented cross-species effects on longevity, fertility, and regeneration in mammals. We describe novel wound healing and stemness maintenance activities for Royalactin, the major protein component in RJ, in mice and in embryonic and post-natal stem cells, respectively. Royalactin activates a pluripotency gene regulatory network through control of chromatin dynamics to affect a phenotype that mimics ground state pluripotency. We also identified, through a haploid genetic screen, heparan sulfate proteoglycans as the major cell surface binding partners for Royalactin in mammalian cells, and demonstrate important functional interactions between Royalactin and well known pluripotency-regulating pathways. Thus, Royalactin can maintain cellular identity through affecting the dynamic state of stem cells.
|
| |
|
| Overall design |
Each experiment was performed in at least biological duplicate with input or other controls when appropriate.
|
| |
|
| Contributor(s) |
Wan DC, Janki PM, Spencley AL, Shankar G, Li TH, Morgan SL, Mariano N, Luo Y, Dana D, Dovey C, Lumb J, Liu L, Brown KV, Luong R, Pitteri S, Wang J, Tu H, Nusse R, Rando TA, Carette JE, Wang KC |
| Citation(s) |
30510260 |
| Submission date |
May 23, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Kevin Wang |
| E-mail(s) |
kevwang@stanford.edu
|
| Organization name |
Stanford University
|
| Department |
Dermatology
|
| Street address |
269 Campus Dr, CCSR 2115a
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (34)
|
|
| Relations |
| BioProject |
PRJNA322555 |
| SRA |
SRP075584 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE81799_RAW.tar |
3.1 Gb |
(http)(custom) |
TAR (of BW) |
| GSE81799_normalized_counts.txt.gz |
331.4 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...