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Series GSE81799 Query DataSets for GSE81799
Status Public on Dec 24, 2018
Title Maintenance of Mammalian Stem Cell States by Honeybee Royalactin
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Royal Jelly (RJ) is the queen-maker for the honeybee Apis mellifera, and has documented cross-species effects on longevity, fertility, and regeneration in mammals. We describe novel wound healing and stemness maintenance activities for Royalactin, the major protein component in RJ, in mice and in embryonic and post-natal stem cells, respectively. Royalactin activates a pluripotency gene regulatory network through control of chromatin dynamics to affect a phenotype that mimics ground state pluripotency. We also identified, through a haploid genetic screen, heparan sulfate proteoglycans as the major cell surface binding partners for Royalactin in mammalian cells, and demonstrate important functional interactions between Royalactin and well known pluripotency-regulating pathways. Thus, Royalactin can maintain cellular identity through affecting the dynamic state of stem cells.
Overall design Each experiment was performed in at least biological duplicate with input or other controls when appropriate.
Contributor(s) Wan DC, Janki PM, Spencley AL, Shankar G, Li TH, Morgan SL, Mariano N, Luo Y, Dana D, Dovey C, Lumb J, Liu L, Brown KV, Luong R, Pitteri S, Wang J, Tu H, Nusse R, Rando TA, Carette JE, Wang KC
Citation(s) 30510260
Submission date May 23, 2016
Last update date May 15, 2019
Contact name Kevin Wang
Organization name Stanford University
Department Dermatology
Street address 269 Campus Dr, CCSR 2115a
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (34)
GSM2175601 -LIF_1(RNA-Seq)
GSM2175602 -LIF_2(RNA-Seq)
GSM2175603 -LIF+RA_1(RNA-Seq)
BioProject PRJNA322555
SRA SRP075584

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE81799_RAW.tar 3.1 Gb (http)(custom) TAR (of BW)
GSE81799_normalized_counts.txt.gz 331.4 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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