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Series GSE81686 Query DataSets for GSE81686
Status Public on Aug 08, 2016
Title Differentiation and specification of resident tissue macrophages [SMART-Seq2]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Tissue resident macrophages are functionally diverse cells that share an embryonic mesodermal origin. However, the mechanism(s) that control their specification remain unclear. We performed transcriptional, molecular and in situ spatio-temporal analyses of macrophage development in mice. We report that Erythro-Myeloid Progenitors generate pre-macrophages (pMacs) that simultaneously colonize the head and caudal embryo from embryonic day (E)9.5 in a chemokine-receptor dependent manner, to further differentiate into tissue F4/80+ macrophages. The core macrophage transcriptional program initiated in pMacs, is rapidly diversified in early macrophages as expression of transcriptional regulators becomes tissue-specific. For example, the preferential expression of the transcriptional regulator Id3 initiated in early fetal liver macrophages appears critical for Kupffer cell differentiation, as inactivation of Id3 causes a selective Kupffer cell deficiency that persists in adults. We propose that colonization of developing tissues by differentiating macrophages is immediately followed by their specification as they establish residence, hereby generating the macrophage diversity observed in post-natal tissues.
Overall design RNA-sequencing of sorted macrophage cell populations (Mac) and progenitors (EMP, pMac) from various tissues and collected at different time points, including technical and biological replicates
Web link
Contributor(s) Mass E, Ballesteros I, Farlik M, Halbritter F, Gunther P, Crozet L, Jacome-Galarza CE, Klughammer J, Kobayashi Y, Gomez-Perdiguero E, Schultze J, Beyer M, Bock C, Geissmann F
Citation(s) 27492475
Submission date May 20, 2016
Last update date May 15, 2019
Contact name Christoph Bock
Organization name CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences
Street address Lazarettgasse 14
City Vienna
ZIP/Postal code 1090
Country Austria
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (93)
GSM2170349 bra mac e10.25_1
GSM2170350 bra mac e10.25_2
GSM2170351 bra mac e10.25_3
This SubSeries is part of SuperSeries:
GSE81774 Differentiation and specification of resident tissue macrophages
BioProject PRJNA322311
SRA SRP075476

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE81686_macrophages_bulk.csv.gz 20.3 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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