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Series GSE81669 Query DataSets for GSE81669
Status Public on Aug 24, 2017
Title DNase Hypersensitivity Peaks from Rat Schwann Cells
Organism Rattus norvegicus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We utilized DNase-Seq to identify open chromatin regions within Schwann cells. This data was used in conjunction with sequence conservation and transcription factor binding site prediction to identify putative cis-regulatory elements critical for Schwann cells. We used three independent biological replicates, and for our studies used sample 'barcode4' as a representative sample for all three. DNase HSS peaks were identified using F-Seq and HOMER, and overlapped to prioritize candidate regions. These regions were then verified using luciferase assays.
Overall design Performed DNase HSS on three independent biological replicates of S16 rat Schwann cells
Contributor(s) Law WD, Antonellis A
Citation(s) 27821050
Submission date May 20, 2016
Last update date May 15, 2019
Contact name Anthony Antonellis
Organization name University of Michigan
Department Human Genetics
Street address 1241 E Catherine St.
City Ann Arbor
State/province MI
ZIP/Postal code 48104
Country USA
Platforms (1)
GPL14844 Illumina HiSeq 2000 (Rattus norvegicus)
Samples (3)
GSM2166058 WT-1 DNase-seq
GSM2166059 WT-2 DNase-seq
GSM2166060 WT-3 DNase-seq
BioProject PRJNA322264
SRA SRP075452

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Supplementary file Size Download File type/resource
GSE81669_RAW.tar 9.8 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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