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Series GSE81467 Query DataSets for GSE81467
Status Public on May 09, 2017
Title The transcription factor Gli3 promotes B cell development in the fetal liver through repression of Shh
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Before birth B-cells develop in the fetal liver (FL). Here we show that Gli3 activity in the FL stroma is required for B-cell development. In the Gli3-deficient FL B-cell development was reduced at multiple stages, whereas the Sonic hedgehog (Shh)-deficient FL showed increased B-cell development, and Gli3 functioned to repress Shh transcription. Use of a transgenic Hedgehog (Hh)-reporter mouse showed that Shh signals directly to developing B-cells, and that Hh pathway activation was increased in developing B-cells from Gli3-deficient fetal liver. RNAsequencing confirmed that Hh-mediated transcription is increased in B-lineage cells from Gli3-deficient FL, and showed that these cells expressed reduced levels of B-lineage transcription factors and BCR/pre-BCR-signalling genes. We showed that expression of the master regulators of B-cell development, Ebf1 and Pax5, is reduced in developing B-cells from Gli3-deficient FL and increased in Shh-deficient FL, and that in vitro Shh-treatment or neutralisation can repress or induce their expression respectively.
Overall design Wildtype and Gli3 mutant (Gli3+/- and Gli3-/-) (n=2) embryonic day 17.5 fetal livers were sorted for CD19+B220+ cells. RNA extracted from these cells was sequenced to help understand the transcriptional changes governing B cell development in the Gli3 mutants.
Contributor(s) Crompton T, Solanki A
Citation(s) 28533268
Submission date May 16, 2016
Last update date May 15, 2019
Contact name Tessa Crompton
Organization name UCL
Department Immunobiology
Street address 30 Guilford Street
City London
ZIP/Postal code WC1N 1EH
Country United Kingdom
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (6)
GSM2152840 WildType Rep1
GSM2152841 WildType Rep2
GSM2152842 Gli3+/- Rep1
BioProject PRJNA321725
SRA SRP075207

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Supplementary file Size Download File type/resource
GSE81467_abundance_values.csv.gz 635.2 Kb (ftp)(http) CSV
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