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| Status |
Public on Nov 07, 2016 |
| Title |
SOX7 supresses the expression of RUNX1 target genes during EHT |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The molecular mechanisms regulating endothelial to hematopoietic transition (EHT) of hemogenic endothelium (HE) are poorly understood. Here we profile the transcriptional changes resulting from SOX7 overexpression during EHT
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| Overall design |
FLK1+ cells were sorted from day 3.5 iSox7 EBs and cultured in liquid blast media for 48hours. Dox was added for 6, 12 and 24 hours to induce SOX7 expression, before samples were harvested for RNAseq.
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| |
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| Contributor(s) |
Lilly A, Fadlullah MZ, Kouskoff V |
| Citation(s) |
27802172 |
| |
| Submission date |
May 16, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Andrew Joshua Lilly |
| E-mail(s) |
zaki.fadlullah@cruk.manchester.ac.uk
|
| Organization name |
CRUK Manchester Institute
|
| Department |
Stem Cell Haematopoiesis
|
| Street address |
Wilmslow Road
|
| City |
Manchester |
| ZIP/Postal code |
M20 4BX |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA321724 |
| SRA |
SRP075208 |
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