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Series GSE8139 Query DataSets for GSE8139
Status Public on Dec 01, 2008
Title Expression data from MCF7/HER2-18 xenografts
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To investigate molecular mechanisms of resistance, we used two different in vivo xenograft models of estrogen receptor-positive (ER+) breast cancer, with or without HER2 over-expression (MCF7/HER2-18 and MCF7 wt, respectively). Mice with established tumors were assigned to the following treatment groups: continued estrogen supplementation (E2), estrogen deprivation (ED), ED plus tamoxifen (Tam), all with or without the EGFR tyrosine kinase inhibitor gefinitinib (G). Another group received ED plus the antiestrogen fulvestrant (MCF7 wt only). Tumors with acquired or de novo resistance to these endocrine therapies were profiled for mRNA expression using Affymetrix Genechip arrays.
Keywords: multiple group comparison
 
Overall design MCF7 xenografts were established in ovariectomized five to six week-old nu/nu athymic nude mice supplemented with 0.25 mg 21 day release estrogen pellets by inoculating subcutaneously (s.c.) 5E-6 cells. When tumors reached the size of 150-200 mm3 (3-5 weeks), the animals were randomly allocated to continued estrogen (E2), continued estrogen with gefitinib (E2+G; 100mg/kg, 5 days/week), estrogen withdrawal alone (ED; by removal of the estrogen pellets), and estrogen withdrawal plus tamoxifen citrate (Tam; 500 microg/animal s.c. in peanut oil, 5 days/week), with either gefitinib (Tam+G; 100mg/kg, 5 days/week) or vehicle (1% Tween 80) administered via gavage, as well as estrogen withdrawal plus fulvestrant (ICI 182,780) in the MCF7 wt model (Fulv; 5mg/mouse s.c. once weekly), and estrogen withdrawal with gefitinib (ED+G). Tumors were harvested for molecular studies when they became resistant to treatment and reached the size of 1000 mm3 (n=7).
 
Contributor(s) Creighton C, Massarweh S, Schiff R, Osborne K
Citation(s) 18794137
Submission date Jun 15, 2007
Last update date Mar 25, 2019
Contact name Chad Creighton
E-mail(s) creighto@bcm.tmc.edu
Organization name Baylor College of Medicine
Department Biostatistics, Ducan Cancer Center
Street address One Baylor Plaza, Mail Stop: BCM305
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (51)
GSM201588 (+) E2 Big Group MCF7/HER2-18 xenograft, biological rep1
GSM201589 (+) E2 Big Group MCF7/HER2-18 xenograft, biological rep2
GSM201590 (+) E2 Big Group MCF7/HER2-18 xenograft, biological rep3
This SubSeries is part of SuperSeries:
GSE8141 Expression data from MCF7 wt and MCF7/HER2-18 xenografts
Relations
BioProject PRJNA105409

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8139_MCF7_HER2-18_z-score_norm.txt 36.4 Mb (ftp)(http) TXT
GSE8139_RAW.tar 407.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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