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| Status |
Public on May 30, 2018 |
| Title |
Comparison of gene expression in macrophages tolerised by different toll-like receptor ligands |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To investigate the similarity of toll-like receptor tolerance in macrophages stimulated with different toll-like receptor ligands we stimulated naïve or tolerant macrophages with ligands for TLR4, TLR2, TLR3 and TLR9. The data identifies a core set of genes that are tolerised by all ligands and genes that show TLR specific patterns.
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| Overall design |
Bone marrow derived macrophages were stimulated with four different toll-like receptor ligands; LPS, CpG, Pam3csk4 and PolyI:C. In addition some cells received a pre-treatment with the same ligand prior to restimulation to induce a tolerant state. Gene expression was measured at 4 hours post stimulation with each ligand. Three independent experiments were performed to measure gene expression changes between each macrophage group. Of the 27 samples, LPSTolerance_3 and Pam3csk4_2 were excluded from the analysis entirely due to low sample quality – they are included in this dataset for completeness only.
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| Web link |
https://www.stemformatics.org/datasets/view/6943
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| Contributor(s) |
Carmody R |
| Citation(s) |
29867935 |
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| Submission date |
May 10, 2016 |
| Last update date |
Feb 27, 2019 |
| Contact name |
Othmar Korn |
| Organization name |
Australian Institute for Bioengineering and Nanotechnology
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| Street address |
University of Queensland
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| City |
St. Lucia |
| State/province |
Queensland |
| ZIP/Postal code |
4067 |
| Country |
Australia |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (27)
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| GSM2149826 |
LPS acute, rep1 |
| GSM2149827 |
LPS acute, rep2 |
| GSM2149828 |
LPS acute, rep3 |
| GSM2149829 |
LPS tolerance, rep1 |
| GSM2149830 |
LPS tolerance, rep2 |
| GSM2149831 |
LPS tolerance, rep3 |
| GSM2149832 |
CpG acute, rep1 |
| GSM2149833 |
CpG acute, rep2 |
| GSM2149834 |
CpG acute, rep3 |
| GSM2149835 |
CpG tolerance, rep1 |
| GSM2149836 |
CpG tolerance, rep2 |
| GSM2149837 |
CpG tolerance, rep3 |
| GSM2149838 |
Pam3csk4 acute, rep1 |
| GSM2149839 |
Pam3csk4 acute, rep2 |
| GSM2149840 |
Pam3csk4 acute, rep3 |
| GSM2149841 |
Pam3csk4 tolerance, rep1 |
| GSM2149842 |
Pam3csk4 tolerance, rep2 |
| GSM2149843 |
Pam3csk4 tolerance, rep3 |
| GSM2149844 |
Poly IC acute, rep1 |
| GSM2149845 |
Poly IC acute, rep2 |
| GSM2149846 |
Poly IC acute, rep3 |
| GSM2149847 |
Poly IC tolerance, rep1 |
| GSM2149848 |
Poly IC tolerance, rep2 |
| GSM2149849 |
Poly IC tolerance, rep3 |
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| Relations |
| BioProject |
PRJNA321202 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE81291_RAW.tar |
324.5 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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