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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 16, 2016 |
| Title |
Polarized regulatory landscape and Wnt responsiveness underlie Hox activation in embryos |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Other
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| Summary |
Sequential 3’ to 5’ activation of the Hox gene clusters in early embryos is a most fascinating issue in developmental biology. Neither the trigger nor the regulatory elements involved in the transcriptional initiation of the 3’-most Hox genes have been unraveled in any organism. We demonstrate that a series of enhancers, some of which Wnt-dependent, is located within a HoxA 3’ subTAD. This subTAD forms the structural basis for multiple layers of 3’-polarized features, including DNA accessibility and enhancer activation. Deletion of the cassette of Wnt-dependent enhancers proves its crucial role in initial transcription of HoxA at the 3’ side of the cluster.
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| Overall design |
ChIP-sequencing for several antibodies, ATAC-seq and 4C on epiblast stem cells (EpiSCs). Additionally, we performed ATAC-seq on different early stage mouse embryos.
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| Contributor(s) |
Neijts R, Amin S, van Rooijen C, Tan S, Creyghton MP, de Laat W, Deschamps J |
| Citation(s) |
27633012 |
| Submission date |
May 06, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Jacqueline Deschamps |
| E-mail(s) |
j.deschamps@hubrecht.eu
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| Organization name |
Hubrecht Institute
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| Street address |
Uppsalalaan 8
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| City |
Utrecht |
| ZIP/Postal code |
3584CT |
| Country |
Netherlands |
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| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (65)
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| Relations |
| BioProject |
PRJNA320877 |
| SRA |
SRP074483 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE81203_RAW.tar |
13.3 Gb |
(http)(custom) |
TAR (of BEDGRAPH, BW, NARROWPEAK, WIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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