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Series GSE81203 Query DataSets for GSE81203
Status Public on Sep 16, 2016
Title Polarized regulatory landscape and Wnt responsiveness underlie Hox activation in embryos
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Sequential 3’ to 5’ activation of the Hox gene clusters in early embryos is a most fascinating issue in developmental biology. Neither the trigger nor the regulatory elements involved in the transcriptional initiation of the 3’-most Hox genes have been unraveled in any organism. We demonstrate that a series of enhancers, some of which Wnt-dependent, is located within a HoxA 3’ subTAD. This subTAD forms the structural basis for multiple layers of 3’-polarized features, including DNA accessibility and enhancer activation. Deletion of the cassette of Wnt-dependent enhancers proves its crucial role in initial transcription of HoxA at the 3’ side of the cluster.
Overall design ChIP-sequencing for several antibodies, ATAC-seq and 4C on epiblast stem cells (EpiSCs). Additionally, we performed ATAC-seq on different early stage mouse embryos.
Contributor(s) Neijts R, Amin S, van Rooijen C, Tan S, Creyghton MP, de Laat W, Deschamps J
Citation(s) 27633012
Submission date May 06, 2016
Last update date May 15, 2019
Contact name Jacqueline Deschamps
Organization name Hubrecht Institute
Street address Uppsalalaan 8
City Utrecht
ZIP/Postal code 3584CT
Country Netherlands
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (65)
GSM2144605 H3K27ac Wnt3KO 0h
GSM2144606 H3K27ac Wnt3KO Chiron6h
GSM2144607 H3K27ac Wnt3KO Chiron12h
BioProject PRJNA320877
SRA SRP074483

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Supplementary file Size Download File type/resource
GSE81203_RAW.tar 13.3 Gb (http)(custom) TAR (of BEDGRAPH, BW, NARROWPEAK, WIG)
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Raw data are available in SRA
Processed data provided as supplementary file

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