|
| Status |
Public on Apr 24, 2017 |
| Title |
Fam60a defines a embryonic stem cell specific Sin3a/Hdac complex. |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
ChIP coupled with NGS identifies genome-wide binding sites of a ES cells specific Sin3a/Hdac complex.
The aim of these experiments is to study the role of Fam60a in the Sin3a/Hdac complex. ChIP-Seq experiments reveal that Fam60a is required to maintain high levels of Sin3a binding on target genes in mESCs. Depletion of Fam60a causes a drop of Sin3a binding to target sites. Underlining the function of Fam60a in mES cells, ChIP-seq analysis of Sin3a and Fam60a in mouse fibroblasts reveal strikingly low global binding level of Sin3a to its target genes while Fam60a is absent at these sites.
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| |
|
| Overall design |
ChIP-Seq of Sin3a and Fam60a in E14 mESCs and NIH3T3. ChIP-Seq of Sin3a following shRNA-mediated knockdown of Fam60a in mESCs.
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| |
|
| Contributor(s) |
Streubel G, Fitzpatrick DJ, Jammula S, Bracken AP |
| Citation(s) |
28554894 |
| |
| Submission date |
May 03, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Darren John Fitzpatrick |
| E-mail(s) |
fitzpadj@tcd.ie
|
| Organization name |
Trinity College Dublin
|
| Department |
Smurfit Institute of Genetics
|
| Street address |
College Green
|
| City |
Dublin 2 |
| State/province |
Ireland |
| ZIP/Postal code |
Eire |
| Country |
Ireland |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA320432 |
| SRA |
SRP074320 |
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