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Series GSE80661 Query DataSets for GSE80661
Status Public on Apr 26, 2017
Title Non-overlapping control of transcriptome by Promoter and Super-Enhancer-Associated Dependencies
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The relationship between promoter proximal transcription factor-associated gene expression and super-enhancer-driven transcriptional programs are not well-defined. Some level of their distinct genomic occupancy may suggest a mechanism with specific target gene control. We explored the transcriptional and functional interrelationship between E2F transcription factors and BET transcriptional co-activators in multiple myeloma. Global chromatin analysis reveals distinct regulatory axes for E2F and BETs, with E2F predominantly localized to active gene promoters of growth/proliferation genes and BETs disproportionately at enhancer- regulated tissue specific genes. Depletion of E2F selectively down-regulates its regulatory axis and is generally synergistic with BET inhibition. In vivo, combined inhibition of BETs and E2F strongly reduces tumor growth, implicating E2F as a myeloma dependency that is potently leveraged with BET inhibition.
Overall design ChIP-Seq in U266 and MM1.S multiple myeloma cell lines
Contributor(s) Munshi NP, Fulciniti M, Lin CY
Citation(s) 30590042
Submission date Apr 26, 2016
Last update date May 15, 2019
Contact name Nikhil C Munshi
Phone 617-632-4218
Organization name Dana-Farber Cancer Institute
Department Hematologic Oncology
Street address 450 Brookline Avenue
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (8)
GSM2132551 MM1S_DP1
GSM2132552 MM1S_E2F1
BioProject PRJNA319620
SRA SRP073902

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE80661_RAW.tar 771.0 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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