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Series GSE8050 Query DataSets for GSE8050
Status Public on Jun 07, 2007
Title A cryptic VEGF T-cell epitope: Identification and characterization by mass spectrometry and T-cell assays.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The vascular endothelial growth factor A (VEGF) is involved in various physiological processes such as angiogenesis or wound healing but is also crucial in pathological events such as tumor growth. Thus, clinical anti-VEGF treatments have been developed which could already prove to have enormous beneficial effects for cancer patients. In this article we describe the first VEGF-derived CD8+ T-cell epitope. The natural HLA ligand SRFGGAVVR was identified by differential mass spectrometry in two primary renal cell carcinomas (RCC) and was significantly over-presented on both tumor tissues. SRFGGAVVR is derived from a cryptic translated region of VEGF presumably by initiation of translation at the non-classical start codon CUG499. SRFGGAVVR specific T-cells were generated in vitro using peptide loaded dendritic cells or artificial antigen presenting cells. They were identified by HLA tetramer analysis after in vitro stimulation. SRFGGAVVR specific CD8+ T-cells were fully functional T-effector cells, which were able to secrete IFN-gamma upon stimulation and killed tumor cells in vitro. Additionally, we have quantitatively analyzed VEGF mRNA and protein levels in RCC tumor and normal tissue samples by gene chip analysis, qRT-PCR, in situ hybridization, and bead based immuno assay. In the future, T-cells directed against VEGF as a tumor associated antigen may represent a possible way of combining peptide-based anti-VEGF immunotherapy with already existent anti-VEGF cancer therapies.
Keywords: disease state analysis
 
Overall design Two clear cell renal cell carcinomas including autologous normal tissue were analyzed.
 
Contributor(s) Weinzierl AO, Maurer D, Altenberend F, Schneiderhan-Marra N, Klingel K, Schoor O, Wernet D, Joos T, Rammensee H, Stevanovic S
Citation(s) 18381453
Submission date Jun 07, 2007
Last update date Mar 25, 2019
Contact name Andreas Oliver Weinzierl
E-mail(s) andi.weinzierl@web.de
Phone 004970712987647
Fax 00497071295653
Organization name University of Tuebingen
Department Department of Immunology
Lab Rammensee
Street address Auf der Morgenstelle 15
City Tuebingen
ZIP/Postal code 72076
Country Germany
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (4)
GSM198783 RCC099_normal tissue_rep 1
GSM198784 RCC099_tumor tissue_rep 1
GSM198785 RCC110-normal tissue_rep 1
Relations
BioProject PRJNA100877

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8050_RAW.tar 45.2 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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