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Series GSE80410 Query DataSets for GSE80410
Status Public on Oct 11, 2016
Title Characterization of tumor-associated-macrophage of breast cancer patient-derived xenografts
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by array
Summary The aim of this study was to characterize the stroma displayed by different models of breast cancer tumors in mice. For this purpose, transcriptomic and flow cytometry analyses on murine populations were performed in a series of 25 PDXs and 2 most commonly used GEMs (MMTV-PyMT and MMTV-erBb2). Specifically, macrophages from 5 models were sorted and profiled by gene expression and phenotypically characterized by flow cytometry.
Composition of hematopoietic cells, mainly macrophages and granulocytes, varied tumor from tumor. Macrophages were shown to display specific polarization phenotype related to M1/M2 classification and associated with genes implicated in recruitment, invasion and metastasis processes. The heterogeneity of stroma component of the studied models suggests that tumor cells educate their microenvironment to fulfill their needs. This observation therefore supports the relevance of such models for preclinical study purposes.
Overall design Macrophage/monocyte-like cells (EpCAM-CD45+F4/80+) were sorted on a FACS
Contributor(s) Vallerand D, Massonnet G, Kebir F, Gentien D, Maciorowski Z, de la Grange P, Sigal-Zafrani B, Richardson M, Humbert S, Thuleau A, Assayag F, Deplater L, Nicolas A, Scholl S, Marangoni E, Weigand S, Roman-Roman S, Savina A, Decaudin D
Citation(s) 27388901
Submission date Apr 18, 2016
Last update date Apr 18, 2017
Contact name Pierre de la Grange
Organization name GenoSplice technology
Department Paris Biotech Santé
Street address 29, rue du Faubourg Saint-Jacques
City Paris
ZIP/Postal code 75014
Country France
Platforms (2)
GPL11532 [HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version]
GPL11533 [MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version]
Samples (33)
GSM2126075 BC52_EDW_p15_Frag
GSM2126076 BC73_BON_p17_Frag
GSM2126077 BC111_VINC_1_Cell_Tum
BioProject PRJNA318812

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE80410_RAW.tar 122.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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