|
| Status |
Public on Aug 25, 2016 |
| Title |
Smart-seq2 analysis of P17 FACS sorted retinal cells from the Tg(Chx10-EGFP/cre,-ALPP)2Clc or Vsx2-GFP transgenic line |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Three Vsx2-GFP mouse retinas were dissected, dissociated and FACS sorted, and single cell RNA-seq libraries were generated for 288 single cells and 3 bulk libraries using Smart-seq2 (~10,000 cells each)
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| |
|
| Overall design |
Three mouse retinas (labeled 1a, 2a, 2b) were used, and 96 single cells from each were processed using Smart-seq2. A bulk RNA-seq control of 10,000 cells from each retina was also prepared
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| |
|
| Contributor(s) |
Shekhar K |
| Citation(s) |
27565351 |
| Submission date |
Apr 13, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Karthik Shekhar |
| E-mail(s) |
karthik@broadinstitute.org
|
| Phone |
217-979-9852
|
| Organization name |
Broad Institute
|
| Street address |
415 Main St.
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (291)
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| This SubSeries is part of SuperSeries: |
| GSE81905 |
Single cell RNA-sequencing of retinal bipolar cells |
|
| Relations |
| BioProject |
PRJNA318366 |
| SRA |
SRP073242 |
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