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Series GSE80231 Query DataSets for GSE80231
Status Public on Apr 28, 2016
Title Sp7/Osterix is restricted to bone-forming vertebrates where it acts as a Dlx co-factor in osteoblast specification [ChIP-Seq 2]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary To identify Sp7’s molecular interactions in vivo, we used gene-targeting strategies to generate an Sp7-Biotin-3xFLAG knock-in mouse (Sp7-BioFL mouse). This approach appends a biotin (Bio) recognition motif and three copies of the FLAG (FL) epitope at the C-terminus of the Sp7 protein. To validate this knockin system, we compared ChIP-seq results in the MC3T3E1 osteoblast cell line, introducing Sp7 forms that were epitope-tagged at either the N- (N-terminal FLAG tag; MC3_FLAG-Sp7) or C-terminus (C-terminal Biotin-FLAG tag, as in the in vivo targeted allele; MC3_Sp7-BioFLAG). These data show an extensive overlap, suggesting that FLAG tagging at different positions gives comparable outcomes. Second, we examined ChIP on wild-type calvarial primary osteoblasts (POB) using an anti-Sp7 antibody (POB_Sp7Ab-ChIP). The majority of Sp7-Ab ChIP-seq peaks (95%) overlapped with the larger set identified by Sp7-BioFL ChIP-seq.
Overall design ChIP-seq experiments with primary osteoblasts and a osteoblast cell line were performed
Contributor(s) Hojo H, Ohba S, He X, Lai LP, McMahon AP
Citation(s) 27134141
Submission date Apr 13, 2016
Last update date May 15, 2019
Contact name Hironori Hojo
Phone +81-3-5841-1427
Organization name The University of Tokyo
Department Center for Disease Biology and Integrative Medicine
Lab Clinical Biotechnology
Street address 7-3-1 Hongo
City Bunkyo-ku
State/province Tokyo
ZIP/Postal code 113-8655
Country Japan
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (5)
GSM2121288 MC3_FLAG-Sp7
GSM2121289 MC3_Sp7-BioFLAG
GSM2121290 POB_Sp7Ab-ChIP
This SubSeries is part of SuperSeries:
GSE76187 Sp7/Osterix is restricted to bone-forming vertebrates where it acts as a Dlx co-factor in osteoblast specification
BioProject PRJNA318364
SRA SRP073243

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE80231_RAW.tar 380.0 Kb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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