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Series GSE79988 Query DataSets for GSE79988
Status Public on Dec 31, 2017
Title The myelin protein PMP2 is regulated by SOX10 and drives melanoma cell invasion
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The transcription factor SRY(sex related protein-Y)-box10 (SOX10) plays a key role in the development of melanocytes and peripheral glial cells from neural crest precursors. Recently, we and other groups found SOX10 to be involved in melanoma initiation, proliferation, invasion, and survival. However, specific mediators which impart the oncogenic role of SOX10 in melanoma remain widely unknown. To identify potential target genes of SOX10, we performed RNA sequencing to analyze genome-wide expression alterations after ectopic expression of SOX10. Among nine genes differentially regulated by SOX10, only peripheral myelin protein 2 (PMP2) was found upregulated in several other melanoma cell lines. PMP2 is one of the most abundant myelin proteins in glial cells and is necessary for the formation and maintenance of the myelin sheath. We detected PMP2 expression in a subset of human melanoma cell lines while it was absent in human melanocytes and fibroblasts. Direct binding of SOX10 to the PMP2 promoter was shown by chromatin immunoprecipitation and electrophoretic shift assay. In three-dimensional spheroid assays, we found that PMP2 overexpression increased melanoma cell invasion. In conclusion, we identified PMP2 as target gene of SOX10 and propose a novel role for PMP2 in melanoma cell invasion.
Overall design RNA sequencing was performed with 1205Lu metastatic melanoma cells transiently transfected with a vector for SOX10 overexpression (pCMV6-SOX10) or a control vector (pCMV6) in three biological replicates.
Contributor(s) Graf SA, Krebs S, Kammerbauer C, Besch R, Berking C
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Submission date Apr 06, 2016
Last update date May 15, 2019
Contact name Stefan Krebs
Phone 0049-89-2180 76715
Organization name Ludwig-Maximilian University
Department Gene Center
Lab Lafuga Genomics
Street address Feodor-Lynen-Str. 25
City Munich
ZIP/Postal code 81377
Country Germany
Platforms (1)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
Samples (6)
GSM2109663 s1
GSM2109664 s2
GSM2109665 s3
BioProject PRJNA317571
SRA SRP072941

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Supplementary file Size Download File type/resource
GSE79988_counts.txt.gz 197.8 Kb (ftp)(http) TXT
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