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Series GSE79818 Query DataSets for GSE79818
Status Public on Jul 13, 2016
Title Microglia development follows a stepwise program to regulate brain homeostasis - Single Cell RNA-seq
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Microglia play important roles in life-long brain maintenance and in pathology, but are also crucial in the developing central nervous system; yet their regulatory dynamics during development have not been fully elucidated. Genome-wide chromatin and expression profiling coupled with single-cell transcriptomic analysis throughout development reveal that microglia undergo three temporal developmental stages in synchrony with the brain: early, pre-, and adult microglia, which are under the control of distinct regulatory circuits. Knockout of the transcription factor MafB caused disruption of homeostasis in adulthood and increased inflammation. Environmental perturbations, such as the microbiome or prenatal immune activation, led to dysregulation of the developmental program, particularly in terms of inflammation. Together, our work identifies a stepwise developmental program of microglia integrating immune response pathways that may be associated with several neurodevelopmental disorders.
Overall design Yolk sac progenitors (CD45+CD11B+CX3CR1-GFP+), microglia from early brain (CD45+CD11B+CX3CR1-GFP+), and microglia from later stages (CD45intCD11BintCX3CR1-GFP+) were isolated from CX3CR1+ C57BL/6J mice
Contributor(s) Amit I
Citation(s) 27338705
Submission date Apr 01, 2016
Last update date May 15, 2019
Contact name Ido Amit
Phone 972-8-9343338
Organization name Weizmann Institute of Science
Department Immunology
Street address 234 Herzl st.
City Rehovot
ZIP/Postal code 760001
Country Israel
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (18)
GSM2104296 AB335 (Sample 1)
GSM2104297 AB336 (Sample 2)
GSM2104298 AB397 (Sample 3)
This SubSeries is part of SuperSeries:
GSE79819 Microglia development follows a stepwise program to regulate brain homeostasis
BioProject PRJNA317060
SRA SRP072734

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Supplementary file Size Download File type/resource
GSE79818_experimental_design.txt.gz 28.7 Kb (ftp)(http) TXT
GSE79818_umitab.txt.gz 3.4 Mb (ftp)(http) TXT
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