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Series GSE79812 Query DataSets for GSE79812
Status Public on Jul 13, 2016
Title Microglia development follows a stepwise program to regulate brain homeostasis - RNA seq
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Microglia play important roles in life-long brain maintenance and in pathology, but are also crucial in the developing central nervous system; yet their regulatory dynamics during development have not been fully elucidated. Genome-wide chromatin and expression profiling coupled with single-cell transcriptomic analysis throughout development reveal that microglia undergo three temporal developmental stages in synchrony with the brain: early, pre-, and adult microglia, which are under the control of distinct regulatory circuits. Knockout of the transcription factor MafB caused disruption of homeostasis in adulthood and increased inflammation. Environmental perturbations, such as the microbiome or prenatal immune activation, led to dysregulation of the developmental program, particularly in terms of inflammation. Together, our work identifies a stepwise developmental program of microglia integrating immune response pathways that may be associated with several neurodevelopmental disorders.
Overall design Yolk sac progenitors (CD45+CD11B+CX3CR1-GFP+), microglia from early brain (CD45+CD11B+CX3CR1-GFP+), and microglia from later stages (CD45intCD11BintCX3CR1-GFP+) were isolated from CX3CR1+ C57BL/6J mice or microglia from perturbation models (CD45intCD11Bint) from mice of C57BL/6J background
Contributor(s) Amit I
Citation(s) 27338705
Submission date Apr 01, 2016
Last update date May 15, 2019
Contact name Ido Amit
Phone 972-8-9343338
Organization name Weizmann Institute of Science
Department Immunology
Street address 234 Herzl st.
City Rehovot
ZIP/Postal code 760001
Country Israel
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (91)
GSM2104025 brain_E12.5 (Sample 1)
GSM2104026 brain_E12.5 (Sample 2)
GSM2104027 brain_E10.5 (Sample 3)
This SubSeries is part of SuperSeries:
GSE79819 Microglia development follows a stepwise program to regulate brain homeostasis
BioProject PRJNA317062
SRA SRP072732

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE79812_GermFree.xlsx 3.5 Mb (ftp)(http) XLSX
GSE79812_MafbKO.xlsx 2.5 Mb (ftp)(http) XLSX
GSE79812_PolyIC.xlsx 3.6 Mb (ftp)(http) XLSX
GSE79812_Timeline.xlsx 4.4 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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