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Series GSE79734 Query DataSets for GSE79734
Status Public on May 01, 2016
Title Epigenetic Profile in RBPJ Maintains Brain Tumor Initiating Cells through CDK9-mediated Transcriptional Elongation
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Glioblastomas display hierarchies with self-renewing cancer stem-like cells (CSCs). RNA sequencing and enhancer mapping revealed regulatory programs unique to CSCs causing upregulation of the iron transporter transferrin, the top differentially expressed gene compared to tissue-specific progenitors. Direct interrogation of iron uptake demonstrated CSCs potently extract iron from the microenvironment more effectively than other tumor cells. Systematic interrogation of iron flux determined that CSCs preferentially require transferrin receptor and ferritin - two core iron regulators - to propagate and form tumors in vivo. Depleting ferritin disrupted CSC mitotic progression, through the STAT3-FoxM1 regulatory axis, revealing an iron-regulated CSC pathway. Iron is a unique, primordial metal fundamental for earliest life forms, and on which CSCs have an epigenetically programmed, targetable dependence.
Overall design H3K27Ac histone modifications and RBPJ transcription factor binding were mapped by ChIP-seq in Glioblastoma initiating cells
Contributor(s) Xie Q, Liau BB, Miller TE, Bernstein BE, Rich JN
Citation(s) 27322055
Submission date Mar 30, 2016
Last update date May 15, 2019
Contact name Tyler E Miller
Organization name Cleveland Clinic Lerner Research Institute
Department Stem Cell Biology and Regenerative Medicine
Lab Jeremy Rich Lab
Street address 2111 E 96th Street, NE3-256
City Cleveland
State/province Ohio
ZIP/Postal code 44106
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (6)
GSM2101764 3691_RBPJ
GSM2101765 4121_RBPJ
GSM2101766 3691_H3K27Ac
This SubSeries is part of SuperSeries:
GSE79736 RBPJ Maintains Brain Tumor Initiating Cells through CDK9-mediated Transcriptional Elongation
BioProject PRJNA316862
SRA SRP072572

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Supplementary file Size Download File type/resource
GSE79734_RAW.tar 440.0 Kb (http)(custom) TAR (of BED)
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Raw data are available in SRA
Processed data provided as supplementary file

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