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Series GSE79496 Query DataSets for GSE79496
Status Public on Mar 22, 2019
Title DMSO-free chemically defined cryopreservation of human mesenchymal stem cells and chondrocytes
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Serum- and xeno-free cryopreservation is suitable for cells to be used in clinical applications. However, dimethyl sulfoxide (DMSO), which is often used in conventional cryoprotectant medium, has been shown to be toxic to many cell types and can induce morphological and gene expression changes that can lead to changes in cell function when they are cultured. In order to evaluate this issue in depth, we examined the efficiency of a DMSO-free/serum-free/xeno-free chemically defined cryoprotectant media, STEM-CELLBANKER DMSO-Free, when culturing human mesenchymal stem cells (MSCs) and chondrocytes. Interestingly, the cells cryopreserved in STEM-CELLBANKER DMSO-Free had a similar growth rate, morphology, and global gene expression profile compared to that observed for cells preserved in conventional freezing medium, which contains 10% DMSO and 20% fetal bovine serum. From these data, we therefore propose that STEM-CELLBANKER DMSO-Free may be an effective tool for maintaining the cellular characteristics of MSCs and chondrocytes during cryo-storage prior to clinical application.
Overall design Gene expressions in human mesenchymal stem cells and chondrocytes were measured after freezing and thawing in several kinds of cryopreservation media.
Contributor(s) Onuma Y, Ito Y
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Submission date Mar 22, 2016
Last update date Mar 23, 2019
Contact name Yuzuru Ito
Organization name National Institute of Advanced Industrial Science and Technology (AIST)
Department Biotechnology Research Institute for Drug Discovery
Street address Central 6, Higashi 1-1-1
City Tsukuba
State/province Ibaraki
ZIP/Postal code 3058566
Country Japan
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (40)
GSM2096026 ADSC_P6_4w_control 1
GSM2096027 ADSC_P6_4w_control 2
GSM2096028 ADSC_P6_4w_SCB 1
BioProject PRJNA315995

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE79496_RAW.tar 126.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data provided as supplementary file

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