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Series GSE79352 Query DataSets for GSE79352
Status Public on Jun 16, 2016
Title The dynamic translatome of retinal ganglion cell axons during assembly and maintenance of the mouse visual system
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Local mRNA translation mediates the adaptive responses of axons to extrinsic signals but direct evidence that it occurs in mammalian CNS axons in vivo is scant. We developed an axon-TRAP-RiboTag approach in mouse that allows deep-sequencing analysis of ribosome-bound mRNAs in the retinal ganglion cell axons of the developing and adult retinotectal projection in vivo. The embryonic-to-postnatal axonal translatome comprises an evolving subset of enriched genes with axon-specific roles suggesting distinct steps in axon wiring, such as elongation, pruning and synaptogenesis. Adult axons, remarkably, have a complex translatome with strong links to axon survival, neurotransmission and neurodegenerative disease. Translationally co-regulated mRNA subsets share common upstream regulators, and novel sequence elements generated by alternative splicing that promote axonal mRNA translation. Our results indicate that intricate regulation of compartment-specific mRNA translation in mammalian CNS axons supports the formation and maintenance of neural circuits in vivo.
Overall design The profiling of ribosome-bound mRNAs in mouse retinal ganglion cell axons at 4 different developmental stages
Contributor(s) Shigeoka T, Jung J, Turner-Bridger B, Ok J, Lin JQ, Amieux PS, Jung H, Holt CE
Citation(s) 27321671, 31825839
Submission date Mar 17, 2016
Last update date Dec 31, 2019
Contact name Christine E Holt
Organization name University of Cambridge
Department Department of PDN
Street address Anatomy Bldg., Downing St.
City Cambridge
ZIP/Postal code CB2 3DY
Country United Kingdom
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (28)
GSM2092714 E17_cre_positive_rep1
GSM2092715 E17_cre_positive_rep2
GSM2092716 E17_cre_negative_rep1
BioProject PRJNA315830
SRA SRP072106

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Supplementary file Size Download File type/resource
GSE79352_RAW.tar 126.4 Mb (http)(custom) TAR (of TXT, WIG)
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Raw data are available in SRA
Processed data provided as supplementary file

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