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Series GSE79320 Query DataSets for GSE79320
Status Public on Oct 21, 2016
Title AP-1 and TEAD4 co-operatively promote the vascular program during hemangioblast specification [ChIP-Seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Embryonic blood cell development occurs via well-defined developmental stages which are recapitulated in vitro by differentiation of embryonic stem cells. This process is tightly regulated by the interaction of tissue- specific and ubiquitous transcription factors with the chromatin landscape in response to outside signals. We previously identified binding motifs for the commonly expressed AP-1 transcription factor family in open chromatin regions specific for early stages of blood specification and thus aimed to study the role of AP-1 for hemangioblast differentiation. Here we show that FOS and JUN together bind to and activate a core set of vascular genes in the hemogenic endothelium and that upon global inhibition of AP-1 by expression of a dominant negative FOS peptide the balance between endothelial and hematopoietic fate is shifted towards blood. Moreover, we demonstrate that in the hemogenic endothelium AP-1 is required for de novo binding of TEAD4, a transcription factor connected to Hippo signaling, to vascular genes. Notably, after the endothelial-to-hematopoietic transition TEAD4 binding is no longer persisting. These findings provide novel mechanistic insights into vascular and hematopoietic development.
 
Overall design Examination of transcription binding patterns, gene expression patterns in developing hemogenic and hematopoietic cells with and without AP-1, high-resolution DNaseI accessibility in hemogenic endothelium and hematopoietic progenitors
 
Contributor(s) Obier N, Cauchy P, Assi SA, Gilmour J, Lie-A-Ling M, Hoogenkamp M, Lichtinger M, Noailles L, Cockerill P, Lacaud G, Kouskoff V, Bonifer C
Citation(s) 27802171
Submission date Mar 16, 2016
Last update date May 15, 2019
Contact name Pierre Daniel Cauchy
E-mail(s) cauchy@ie-freiburg.mpg.de
Phone +49 (0)761 5108-730
Organization name Max Planck Institute of Immunobiology and Epigenetics
Department Department of Cellular and Molecular Immunology
Lab Laboratory Rudolf Grosschedl
Street address St├╝beweg 51
City Freiburg
State/province Baden-W├╝rttemberg
ZIP/Postal code 79108
Country Germany
 
Platforms (4)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (7)
GSM2091492 Fos d1BC minus dox
GSM2091493 Jun d1BC WT
GSM2091494 TEAD4 d1BC minus dox
This SubSeries is part of SuperSeries:
GSE79323 AP-1 and TEAD4 co-operatively promote the vascular program during hemangioblast specification
Relations
BioProject PRJNA315457
SRA SRP071904

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE79320_RAW.tar 1.7 Gb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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